The MycoSensor QPCR Assay Kit is used to detect Mycoplasma infection of cell cultures by real-time quantitative PCR (QPCR), using SYBR® Green dye detection. The MycoSensor QPCR assay kit includes a primer mix that amplifies the eight most common species of Mycoplasma that infect cell cultures and a convenient master mix containing the components necessary for PCR amplification and fluorescence detection.
The MycoSensor QPCR assay kit detects the following Mycoplasma and Acholeplasma species, which make up the eight most commonly-encountered agents of tissue culture infections: M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. pirum, M. salivarium, M. orale and A. laidlawi. Although detection of other, less common species has not been specifically tested, the assay may detect the presence of other related species.
The MycoSensor QPCR assay kit primer mix is capable of detecting Mycoplasma infections in cell cultures in less than two hours, depending on the spectrofluorometric thermal cycler used for detection. The Mycoplasma primer mix, used in the polymerase chain reaction (PCR), can detect most Mycoplasma infections using template from as little as 100 µl of cell culture supernatant. Cell-growth-inhibiting or weak Mycoplasma infections can be detected by testing extracts made directly from cells.
Detection of M.orale genomic DNA Using MycoSensorQPCR Assay Kit. The MycoSensor QPCR Assay Kit detects a range from 100,000 copies to 10 copies of M.orale genomic DNA in the presence of HeLa cell culture supernatant. The reactions were done in duplicate on the Mx3000P system and the amplification plots are shown here.
For Research Use Only. Not for use in diagnostic procedures