The Mx3000P system is amoung the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies. Highly reproducible results are the product of the Mx3000P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy. The advanced optical system used by the Mx3000P offers numerous benefits:
Eliminates optical variation by ensuring uniform excitation intensity and emission measurement from well-to-well, across the entire plate. Elimination of optical variation comes from the single-scanning fi ber optic head design that ensures:
Each well receives the same amount of excitation light
Each well receives excitation light for the same length of time
Each well is the same distance from the detector
Eliminates the need for well-to-well signal correction by calibration or reference dyes. Uniformity of well-to-well measurements from the single-scanning fi ber optic head design reduces the need for additional signal correction and calibration steps, streamlining your assay.
Allows accurate quantifi cation of low- to high-abundance targets. Use of a single photomultiplier tube (PMT) that provides linear detection over ten orders of magnitude ensures superior sensitivity and reproducibility.
Increases flexibility. Four optical channels with user-selected filters that cover a broad range of excitation wavelengths, allowing use with most fluorescent dyes
Mx3000P QPCR System—Full-Featured Performance
The Mx3000P is a high-performance yet low-cost option designed to accommodate basic to advanced experimental applications. Featuring four optical channels with user-selected filters covering a broad range of excitation wavelengths, the Mx3000P can be used with most fluorescent dyes, bringing flexible real-time qPCR instrumentation to the individual researcher with a limited budget.
Excitation source: Quartz Tungsten Halogen lamp
Detector: 1 scanning photomultiplier tube (PMT)
Multiplexing capabilities: 4 targets in the same tube
Thermal system: solid-state, Peltier-based
Embedded computer safeguards data
Tube/plate formats: 200 µL , 96-well format, optimized for 25 µL sample size
Filters: Dual customizable filter wheels with four user-selected filters*
Dynamic Range: 10 orders of magnitude
Excitation Range: 350nm-750nm
Emission Range: 350nm-700nm
Temperature Uniformity: +/- .25°C at 72˚C
Temperature Ramp Rate: up to 2.5°C/second
Temperature Range: 25-99°C
Dimensions: 14¨ (35.6 cm) W x 20¨ (50.8 cm) D x 18¨ (45.7 cm) H, weight: 45 lbs (20.4 kg)
*Choice of filters includes: ALEXA Fluor 350 (350nm-440nm), FAM/SYBR® Green I (492nm-516nm), TET (517nm-538nm), HEX/JOE/VIC (535nm-555nm), Cy3 (545nm-568nm), TAMRA (556nm-580nm), ROX/Texas Red (585nm-610nm), Cy5 (635nm-665nm), ATTO (440nm-492nm), and HYB Probes (492nm-635nm).
For Research Use Only. Not for use in diagnostic procedures