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MVP Human Cell Line Total RNA - Details & Specifications

The quality of the RNA is assessed visually by observing distinct 28S and 18S ribosomal bands on a non-denaturing gel. Purity of the RNA is assessed by spectrophotometry (A260/A280 > 1.8). RNA is further shown to be free of contaminating RNases by incubation in a suitable buffer at 37ºC.

Total RNA and Poly(A)+ RNA products are subjected to stringent quality assurance functional testing, including Northern analysis and RT-PCR.

Products were isolated using a modified guanidinium thiocyanate method. Human tissue was obtained using protocols approved by an Institutional Review Board. Total RNA is enriched for mRNA by two successive rounds of Oligo(dT)-cellulose selection to produce our Poly(A)+ RNA.

Applications
  • Northern analysis
  • cDNA synthesis
  • QRT-PCR
  • In vitro translation
  • Ribonuclease protection assay
  • S1 nuclease analysis
  • Microarray gene expression analysis
  • For Research Use Only. Not for use in diagnostic procedures