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DNase I, RNase Free - Details & Specifications

Feature Description
Unit Definition: One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume.
Concentration: 10 U/μl
Purity: Incubation of 100-fold molar excess DNase I with RNA for one hour at 37°C produces no noticeable RNA degradation upon analysis by denaturing polyacrylamide gel electrophoresis.
Reaction Conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50% glycerol (v/v). (Dilutions should be made in this buffer.)

For Research Use Only. Not for use in diagnostic procedures