## Competent Cells for Toxic Clones - Details & Specifications

The ABLE C and ABLE K strains are designed to enhance the probability of retrieving clones that are toxic to *E. coli* through the modulation of plasmid copy number. This copy number reduction decreases the levels of cloned gene products and enhances the probability that a toxic clone will be propagated. Positive clones observed on initial screening of lambda libraries can be excised or re-cloned into any convenient vector and can be introduced into the ABLE strains.

The ABLE C strain reduces the copy number of common cloning vectors (any ColE1-derived vector) by ~4-fold compared to the XL1-Blue strain. **Efficiency:** 5 x 10^{6} - 2 x 10^{7} transformants/µg of pUC18 DNA

Electroporation-competent version of ABLE C Competent Cells.**Efficiency:** 5 x 10^{9} transformants/µg of pUC18 DNA**Resistant to kanamycin and tetracycline**.

The ABLE K strain reduced the copy number of common cloning vectors (any ColE1-derived vector by ~10-fold compared to the XL1-Blue strain.

Electroporation-competent version of ABLE K Competent Cells.**Efficiency: ** 5 x 10^{9} transformants/µg of pUC18 DNA

*For Research Use Only. Not for use in diagnostic procedures*