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Brilliant III SYBR Master Mixes - Details & Specifications

Brilliant III Ultra-Fast SYBR® Green QPCR Master Mixes employ a novel mutant of Taq DNA polymerase that exhibits higher nucleotide incorporation rate and a faster-activating chemical hotstart to ensure high specificity and short denaturation times (Figure 1A & 1B). The enhanced chemical hot start technology improves the specificity and precision of detection over a wide range of target concentrations. These reagents deliver high quality performance, especially with difficult to amplify targets or low abundant genes, resulting in a greater dynamic range and improved sensitivity.. With the Brilliant III Ultra-Fast QPCR Master Mixes, researchers can benefit from the time saving and increased sample throughput without compromising the quality of their qPCR results.

Highly efficient one-step QRT-PCR is performed with our Brilliant III Ultra-Fast QRT-PCR reagents using a Moloney-based RT for 1st strand synthesis with optimal performance at a synthesis temperature of 50°C.

AffinityScript QPCR cDNA Synthesis Kit can be used for 1st strand cDNA synthesis in a 2-step providing flexibility across a wide range of temperatures. Novel hotstart Taq DNA polymerase combined with AffinityScript RT, minimizes the potential for primer-dimer formation or other non-specific PCR products and delivers the most reproducible results.


Figures 1A & 1B. Minimizing Primer Dimerization Delivers Superior Sensitivity – Five-fold standard curve (A) showing a dilution series of 50 ng to 5 pg of human genomic DNA detecting Numb-1on the BioRad CFX96 real-time PCR system. The standard curve depicts similar efficiencies for Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix compared to competitor master mixes. However, the Dissociation Curve (B) shows primer-dimers or secondary non-specific PCR artifacts for all competitor master mixes except Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix. Company T generates earlier Cts, but the efficiency is compromised by formation of these artifacts which can compete with the specific product. The lack of such artifacts from the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix reactions suggests that the hot start technology of the reagents prevents generation of such non-specific secondary products, and thereby provides a greater degree of confidence.

Brilliant III Ultra-Fast shortens the total cycling time by ~60% compared to standard cycling kits while maintaining amplification efficiency, R2, dynamic range, and detection sensitivity

Taq42 mutant and faster-activating chemical hot start are the two key components of our newly formulated Brilliant III qPCR master mixes for fast cycling. In the top panel, B7 target (148bp) was amplified (in quadruplicate) from 2-fold dilutions of plasmid DNA at concentrations ranging from 1536 to 3 copies/Rxn. Brilliant III SYBR generates similar amplification efficiency and Rsq values (98.1% and 0.994) compared to Brilliant II SYBR standard cycling kit (95.4% and 0.997). Cqs are also similar (within ±1Cq) for both formulations based on standard curves. Amplifications were performed on Life Technologies StepOnePlus.

In the bottom panel, GAPDH target (151bp) was amplified from 10-fold dilutions of cDNA at concentrations ranging from 500ng to 0.05pg/Rxn. Brilliant III SYBR generates similar amplification efficiency and Rsq values (91% and 0.998) compared to Brilliant II SYBR standard cycling kit (93.8% and 0.996). Cqs are also similar (within ±1Cq) for both formulations based on standard curves.


For Research Use Only. Not for use in diagnostic procedures