SAMPLE PREPARATION PRODUCTS FOR CHROMATOGRAPHY

Reliably extract and concentrate samples from complex matrices

Sample preparation is an essential part of successful chromatography. It extends column lifetime, reduces the need for repeated samples, and minimizes interferences that can jeopardize your separation, detection, and quantification.

Agilent offers the most complete line of sample prep products across the full spectrum of instrumentation. These include:

• Bond Elut SPE products – selectively remove interferences and/or analytes from challenging matrices. They feature trifunctional bonding chemistry for greater stability – plus a three-tier QC process that confirms the correct particle size. Choose from the largest selection of sorbent formats in the market today.

• Pre-packaged QuEChERS kits – make sample preparation faster, easier, and more reliable. Options include extraction kits with pre-weighed salts in anhydrous packets, dispersive kits that accommodate aliquot volumes specified by AOAC/EN methods, and ceramic homogenizers that promote consistent extraction and recovery.

• Filtration products improve both system performance and analytical quality and prevent extractables or other contaminants from damaging the integrity of your samples. Choose from the industry’s widest variety of membrane types and pore sizes to suit your applications.

• Agilent Bond Elut Dried Matrix Spotting cards use an innovative, non-cellulose technology that delivers a new level of confidence in sample collection, with significantly improved analytical sensitivity, reproducibility and ease-of-use.

Agilent Solutions

PUT MORE THAN 40 YEARS OF RELENTLESS INNOVATION
BEHIND YOUR EVERY RESULT

By continually raising the standards for technologies that support your routine analyses, Agilent’s R&D efforts have led to breakthroughs such as:

• New GC columns that help you achieve higher levels of inertness and column-to-column reproducibility

• LC column choices that deliver the sensitivity and reliability you need for demanding applications

• Cutting-edge sample preparation products that promote reliable extraction and concentration

• Fresh atomic and molecular spectroscopy ideas for identifying and confirming targets and unknowns

Longtime Agilent customers have experienced our commitment firsthand. And now, we look forward to demonstrating how Agilent’s approach to relentless innovation can work to your advantage, too.

CHEMICAL ANALYSIS SOLUTIONS

Food

From high-volume screening of vegetables for large numbers of pesticides to rapid identification of pathogens, Agilent understands the analytical needs of food producers, shippers, and regulators. When a new toxin appears, we deploy substantial resources to quickly help customers develop robust and reliable methods. Agilent’s leading separations, mass spectrometry, and spectroscopy solutions are emerging as valuable food testing techniques.

Environmental

Agilent offers more than 40 years of environmental testing and regulatory expertise. We help government and private labs with the full range of assays, from routine testing of soils for heavy metals to detection of pharmaceuticals in groundwater, in concentrations down to parts per trillion.

Energy and Fuels

Agilent collaborates closely with process industry customers to offer analytical systems that meet their needs for separation, detection, throughput, and support. We’ll even preconfigure custom or standard analyzers so they arrive at the lab ready-to-go. Agilent’s expertise in both chemical analysis and life science is a powerful combination for researching and producing biofuels, including a wide range of analytical techniques for fatty acid methyl esters (FAMEs). Our newly-expanded portfolio also offers powerful tools for developing and producing photovoltaic films and solar panels.

Forensics

Because the careers of world class athletes and many other individuals hinge on drug testing, it’s critical that those doing the testing have the highest level of confidence in the results. Forensics analysts worldwide have grown to depend on Agilent tools for accuracy, reliability, and speed in this high stakes, high-throughput field. Our best selling GC, GC/MS and popular LC and LC/MS are workhorses in forensics labs.

Traditional Lab Informatics

The ways labs generate and store data profoundly affect their efficiency. Agilent offers a rich, integrated suite of software products built on a set of customer-driven architectural values with the Agilent OpenLAB Laboratory Software Suite. OpenLAB delivers superior performance, open systems integration and investment protection. Our commitment is to deliver more value across each step in the life cycle of scientific data – from data collection and analysis to interpretation and management.

Materials Science

Agilent offers a newly expanded portfolio of instruments used for the research, manufacturing and testing of advanced materials, from precision optics to pulp, paper and polymers. Tools for chromatography, atomic absorption spectroscopy, molecular spectroscopy, X-ray crystallography, and nuclear magnetic resonance all support continuous progress in materials science.

LIFE SCIENCE SOLUTIONS

Biopharmaceutical

As “multi-omics” studies gain momentum in the search for new therapeutics, Agilent is uniquely positioned to provide the instruments, reagents, and powerful software needed for performing experiments in multiple disciplines and combining the massive amounts of data into biological insight.

Pharmaceutical

Drug manufacturing requires the accuracy, sensitivity and high throughput of other analytical applications, along with the demands of regulatory record-keeping and validation requirements. Agilent provides a potent combination of rugged, high-throughput tools and unmatched compliance services. Agilent now offers the market-leading family of dissolution apparatus and sampling systems that pair perfectly with our HPLC and UV systems.

Proteomics

Research into how large sets of proteins affect the health of an organism requires special sets of analytical tools. Agilent has built a formidable arsenal of liquid chromatograph/mass spectrometers, bioinformatics systems, multiple affinity protein removal columns, and OFFGEL electrophoresis for protein identification and protein biomarker discovery. Accurate-Mass mass spectrometry and the microfluidic HPLC-Chip/MS are two Agilent innovations speeding the work of proteomics researchers around the globe.

Metabolomics

Collections of small molecules are increasingly being seen as rich sources of biomarkers, but studying metabolites presents many challenges. The need for speed, accuracy, and powerful interpretation capabilities in looking at chemical profile snapshots is underscored because molecules are constantly entering, leaving or changing within the metabolome. Agilent’s GC, LC, NMR and MS portfolios, along with our excellent bioinformatics offerings, user-customizable METLIN metabolite database for LC/MS, and the industry’s first commercial GC/MS retention time locked metabolite library align well with needs of metabolomics researchers.

Genomics

Agilent is a global leader in microarrays, scanners, and reagents used in a wide variety of genomic-based disease research experiments. Our SureSelect Target Enrichment System dominates the category, streamlining next generation sequencing studies worldwide. Agilent offers a wide range of catalog microarrays and a highly-developed capability to produce custom arrays featuring ink jet-based SurePrint fabrication and the eArray on-line design tool. All Agilent microarrays feature highly sensitive, selective 60-mer probes. With as many as eight arrays printed on a standard 1 x 3 in. slide, the cost per experiment becomes very affordable.

Life Science Informatics

Mirroring its extensive instrument portfolio, Agilent offers the industry’s most extensive suite of bioinformatics software, helping users derive knowledge from complex genomic, proteomic, metabolomic and other biological data. This includes DNA Analytics for analyzing CGH, ChIP and methylation microarray data. The GeneSpring suite includes informatics software for microarray-based gene expression data, genotyping data, and GeneSpring MS, which are useful for analyzing mass spec data from proteomics and metabolomics experiments and comparing complex datasets to explore biological questions from multiple perspectives.

Lab Automation

To meet the skyrocketing demand for more throughput and automation, Agilent has substantially expanded its lab automation offerings. The Agilent line of liquid handlers and microplate processors are designed to streamline high-volume life science workflows. Agilent is also continually upgrading its advanced autosamplers for LC, GC, LC/MS and GC/MS, adding functionality and speed to reflect the performance of its advanced instruments.

Vacuum Technology

Agilent works with customers to solve vacuum challenges from experiments in high-energy physics to developing systems for producing flat panel displays. Agilent manufactures vacuum systems used in its own mass spectrometry instruments as well as those of other manufacturers. Agilent’s vacuum technology has been proven by the most powerful physics experiment ever built, CERN’s Large Hadron Collider machine, which was used in the discovery of the Higgs boson particle.

Agilent Service and Support for Instrument Systems

Focus on what you do best

For over 40 years, Agilent has been building and maintaining the instruments you count on to stay competitive and successful. Trust us to protect your investment with a broad portfolio of services, backed by a global network of experienced service professionals dedicated to the productivity of your lab.

Get the Agilent Service Guarantee

Should your instrument require service while covered by an Agilent service agreement, we guarantee repair or we will replace your instrument for free.

No other company offers this level of commitment to keep your lab up and running at peak efficiency.

Laboratory decision makers and users ranked Agilent as their first choice for general laboratory compliance services.

Agilent Advantage Service Plans

The best service available for your Agilent instruments

Agilent offers a flexible range of service plans so that you can choose the level of coverage that is best for your lab.

• Agilent Advantage Gold – Priority-one coverage for ultimate uptime and productivity

• Agilent Advantage Silver – Comprehensive coverage for dependable laboratory operations

• Agilent Advantage Bronze – Total repair coverage at a fixed annual price

• Agilent Repair Service – Basic coverage for reliable instrument repair

Agilent Advantage service plans include Agilent Remote Advisor for real-time remote monitoring and diagnostics. Through secure internet connections, you can interact with Agilent service professionals, receive detailed asset reports, and configure text or email alerts to notify you before problems occur – helping you to maximize instrument uptime and optimize laboratory workflows.

Agilent Compliance Services

Equipment qualification that meets the most stringent requirements

Enterprise Edition Compliance was developed to streamline compliance across your entire lab. Used globally in regulated labs, including standards organizations and regulatory agencies, Enterprise Edition enables you to:

• Improve qualification efficiency by automating protocols across platforms to ensure greater efficiency and minimize regulatory risk

• Standardize your entire compliance operation with robust test designs that work with all your instruments

• Add, remove or reconfigure tests based upon your unique user requirements

• Significantly reduce staff review time with consistently formatted, computer generated, tamper-proof reports

Agilent Education and Consulting Services

Our best minds, working for you

Make the most of your instrument with training and consulting from the same experts who designed the instruments, software and processes you use every day.

• Classroom and on-site training in instrument operation, troubleshooting and maintenance

• Customized consulting services to meet your lab’s unique needs

The Agilent Value Promise – 10 Years of Guaranteed Value

In addition to continually evolving products, we offer something else unique to the industry – our 10-year value guarantee. The Agilent Value Promise guarantees you at least 10 years of instrument use from your date of purchase, or we will credit you with the residual value of the system toward an upgraded model. Not only does Agilent ensure a reliable purchase now, but we also ensure that your investment is just as valuable in the future.

For more detailed information,
please go to www.agilent.com/chem/services
or contact your local Agilent Services and Support representative.

Technical Support at work for you

Have a hardware, software, application, instrument repair or troubleshooting question? Agilent’s technical experts are available to answer your questions. With years of laboratory experience, our technical support specialists can provide in-depth knowledge and experience.

For questions pertaining to supplies found in this catalog, contact your local Agilent office or Authorized Agilent Distributor or visit www.agilent.com/chem/techsupport

Need more information?

Visit www.agilent.com/chem/contactus to:

• Locate your nearest Agilent office or distributor for expert technical support.

• Get fast sales and product assistance by phone. Simply use the scroll-down menu to select your country.

• Receive email assistance using our convenient online forms.

FEATURED PRODUCTS

Bond Elut Plexa

Bond Elut Plexa is the next generation of polymeric SPE products. A unique polymeric functionality and optimized methodologies deliver high recoveries with excellent cleanliness, reduced ion suppression and ease-of-use in any SPE workflow.

View Here

Agilent Bond Elut QuEChERS Kits

With Agilent Bond Elut QuEChERS disposable pre-weighed extraction and dispersive kits, you can extract and prepare complex matrices for multi-class, multi-residue pesticide analysis in minutes rather than hours.

View Here

Captiva Filtration

Faster than centrifugation and easily automated, Captiva’s unique dual-depth filtration media provide complete removal of precipitated proteins and outstanding resistance to sample clogging.

View Here

SAMPLE PREPARATION

Option 1 – Interference Guide: Select your Sample Preparation technique based on the type of interferences(s) you need to remove

*Bond Elut DMS cards are for sample collection/transport and are not for sample cleanup

TIPS & TOOLS |
Agilent suggests adding filtration to any sample preparation process to extend the system uptime and maximize your application’s performance. | [picture]

Option 2 - Application Guide: Select the Sample Preparation product best suited for your analysis needs

| TIPS & TOOLS

Simplify your operations with Agilent J&W DB-CLP1 and DB-CLP2 GC columns – the most flexible universal column pair for nine EPA dual-ECD pesticide methods. Together, these fast, reliable columns deliver excellent resolving power with exceptionally low bleed while eliminating the need for time-consuming column switching. Learn more at www.agilent.com/chem/CLP

Option 3 - Sample Preparation Reference Guide: Select the Sample Preparation product best suited for your matrix and compound types

*Dispersive Solid Phase Extraction

Option 4 - Format Guide: Select the Sample Preparation product best suited for your analysis requirements

Agilent Offers a Broad Range of Tube Formats and 96-well Plate Designs

We have a full set of straight barrel tubes ranging from 1-150 mL in a wide range of bonded silica and polymeric chemistries, sorbent particle sizes and bed masses.

For more specialized applications, the Luer compatible Bond Elut Jr and the funnel-shaped large reservoir capacity (LRC) tubes offer flexibility and function across a range of sorbent bed masses. To support automation, tabless (flangeless) versions of the straight-barrel cartridges are also available.

Bond Elut 96-well Plates

Bond Elut 96-well plate formats are best in class for flow performance and well-to-well reproducibility. These specially designed plates are available with well volumes of 1 mL and 2 mL and in a large range of different sorbent chemistries.

VersaPlate

VersaPlate is an innovative, versatile design that lets you customize plates. Insert tubes packed with different phases for sorbent screening, or insert only enough tubes to match the number of samples to be extracted for minimal waste. Luer tip of Versaplate tubes can also fit VacElut 12, VacElut 20, and VacElut SPS 24 vacuum manifolds. VersaPlate can be purchased in a pre-packed 96 position format or as loose tubes.

Packed Formats for Automation

Bond Elut sorbents are also available in packed bed formats for automation platforms, such as the Spark Holland Symbiosis, Gilson ASPEC and Gerstel MPS systems. Agilent’s unique OMIX pipette format is also used with a wide range of liquid handling devices, ranging from hand-held pipettors to high-throughput automated systems.

Solid Phase Extraction (SPE)

Agilent Bond Elut:
Accuracy Starts Here

For over 30 years, Bond Elut has been the most trusted name in solid phase extraction. After years of use, demanding chemists at top companies worldwide have thoroughly documented its many applications and proven its performance.

Bond Elut is manufactured using state-of-the-art automation to guarantee quality and consistency. Optical scanners installed throughout our automated assembly process inspect each Bond Elut tube at multiple points. And during manufacture, 25 different tests are conducted to ensure reproducibility. If an imperfection is spotted, the tube is removed from the assembly line. The result is consistently reliable Bond Elut cartridges, time and time again.

Over 40 different sorbent functionalities are available in a variety of cartridge formats including straight barrel, large reservoir capacity (LRC) and Bond Elut Junior (Jr). 96-well plate configurations support automated workflows, with flexibility for method development and scale-up. Bulk packaging of popular products provides a cost-effective solution for high throughput. Trust integrated solutions from Agilent to connect your sample preparation, analysis and reporting needs to deliver the quality and reliability your lab needs.

The Bond Elut Difference

• Heritage of Reliability: With years of use in some of the most demanding analytical laboratories in the world, Bond Elut products have a proven track record resulting in a strong publication pedigree

• Options for Your Needs: Offering extraction solutions for the widest range of analytes and matrices, bonded silica phases for high specificity methods and polymeric phases for rapid method development, Bond Elut has the largest choice of formats and sorbents in the market today

• Innovative Products Designed for Lab Efficiency: Whether it be fast flow polymeric particles or our patented 96-well plate design, all Bond Elut products are created for ease-of-use, reliability and flexibility to meet both manual and automated requirements

• Technical Support at Every Step: For your specific applications, or to help solve occasional technical issues, a global team of analytical scientists is on hand to assist

• World Class Manufacturing and Quality: Unrivaled manufacturing control, plus exacting ISO 9001: 2000 compliant inspections guarantee the consistent quality of Bond Elut

Cross Reference of Comparable Phases by Manufacturer

Different chemistries and manufacturing processes create sorbents that exhibit differences in selectivity, so there is no universal equalivent for every application. However, the performance of products can be similar in many applications. This table provides suggestions for using Agilent Bond Elut products in comparison to products from other manufacturers.

If you are an Agilent SampliQ user, please contact our Technical Support for Bond Elut options for your sample prep needs.

TIPS & TOOLS |
For additional details on Agilent polymeric SPE products, see the Agilent Bond Elut Plexa and Polymeric SPE Selection Guide, publication number 5990-8589EN. For details on Agilent Silica-Based SPE products, see the Agilent Bond Elut Silica-Based SPE Selection Guide, publication number 5990-8591EN. | [picture]

Particle Size Specifications

You will note that our most common silica-based Bond Elut packings are described as 40 μm materials, yet if you look at the actual lot analyses, you will see that the actual mean is around 55 μm. We have been making silica-based Bond Elut packings since 1979, using the same diameter silicas; in that time, the models used to estimate irregular particle “diameters” and the testing equipment have changed. We have retained the term “40 μm” however, because there are so many official methods that specify a 40 μm Bond Elut sorbent. As other suppliers attempted to copy the successful Bond Elut product specifications, the term has become an industry standard. You can be assured that the actual average particle in our regular silica Bond Elut is the same now as it was 30 years ago when we first pioneered SPE as a sample prep technology.

| TIPS & TOOLS
[picture] | If you don’t see exactly what you’re looking for, Agilent offers custom configurations for many of our sorbents and formats. Requests for custom products can be requested at www.agilent.com/chem/sampleprep or contact technical support at SPP-Support@agilent.com

Bond Elut Plexa Polymeric SPE

The Bond Elut Plexa Family is a new generation of polymeric SPE products, designed for simplicity, improved analytical performance and ease-of-use. Its uniqueness lies in the novel hydroxylated exterior, hydrophobic interior and advanced polymeric architecture.

Bond Elut Plexa

Bond Elut Plexa is a non-polar divinylbenzene-based neutral polymeric sorbent. This sorbent is the best choice for non-ionic extraction of a wide range of acidic, neutral and basic analytes from different matrices.

Bond Elut Plexa PCX

Bond Elut Plexa PCX is a cation exchanger with mixed mode sorbent characteristics and is therefore suitable for the extraction and cleanup of weak bases from biofluids. Bond Elut Plexa PCX demonstrates the same excellent particle size distribution and integrity as Bond Elut Plexa. A highly controlled sulfonation process results in zero fines for Bond Elut Plexa PCX.

Bond Elut Plexa PAX

Bond Elut Plexa PAX is an anion exchange based on the same innovative base polymer particle technology as the other members of the Plexa SPE family. This advanced material offers excellent flow characteristics due to its monodisperse particle size distribution, affording superior ease-of-use, with minimal clogging of the packed bed. The amide-free particle technology does not provide binding sites for endogenous interferences such as proteins and lipids.

Advanced Polymer Architecture Improves Extraction Performance

LOAD:

Water-rich, hydrophilic surface allows excellent phase transfer of analytes into the polymer core.

Large endogenous proteins do not bind to the surface of the polymer and cannot access pore structure.

WASH:

Analytes that have crossed the hydrophilic layers will remain tightly bound in the hydrophobic core.

Interferences wash away without leaching the analytes of interest.

ELUTE:

Specially engineered pore structure allows excellent mass transfer out of the polymer.

Clean extract with high recovery.

General Protocol for Trouble-Free SPE Applications with Bond Elut Plexa Polymeric SPE

Regardless of your application or sample type, you will appreciate the difference the Bond Elut Plexa range makes. Plexa delivers simple methods and superior flow characteristics that effectively eliminate common matrix background that can cause interference and ion suppression, resulting in improved analytical sensitivity and data quality.

Improved Sensitivity

Matrix background can result in significantly decreased analytical sensitivity due to interference, co-elution or ion suppression. Bond Elut Plexa gives you higher recoveries in cleaner extracts, which translates into better sensitivity. Plexa delivers high recoveries regardless of whether absolute or relative calculations are used. This indicates that interference is minimized and maximum sensitivity is achieved. Relative recovery calculations (green bars) are routinely used, but these may mask the effects of interference or ion suppression, which are normalized.

Plexa improves sensitivity by minimizing interference or ion suppression effects and maximizing recovery

Comparison of particle sizes of non-polar SPE polymers by imaging analysis

Comparison of particle size distributions of non-polar SPE sorbents

The narrow particle size distribution offers reproducible, superior flow characteristics with minimal clogging

Bond Elut Plexa

• Fast flow, reproducible performance and ease-of-use

• Improved extract cleanliness minimizes sample matrix interferences

• Non-polar retention mechanism

Bond Elut Plexa polymeric SPE offers simple, easy-to-use methods that simplify sample preparation processes. The water-wettable, hydroxylated exterior allows excellent flow, even with biological fluids. A gradient of polarity on the polymer surface shunts small analytes to the more hydrophobic center of the polymer bead, where they are retained prior to the washing and elution steps. Plexa provides these performance enhancements due to a unique polymeric architecture with a non-retentive, hydroxylated, amide-free surface and a non-polar PS/DVB core for retaining small molecules. Binding of proteins and lipids on the polymer surface is minimized, resulting in cleaner samples and reduced matrix interference. Plexa is ideal for high-throughput tests requiring validated performance with minimal method development. The standard non-polar retention mechanism is applicable to almost any analyte type. The performance features operate at the sample loading step, making them largely method independent.

  Typical Matrices

Plasma, urine, biological fluids and aqueous samples

  Primary Extraction Mechanism

Non-polar

TIPS & TOOLS |
Tabless (flangeless) cartridges are suitable for use with many automated SPE systems. Tabless products are typically designated with a “T” in the part number. If you need a tabless cartridge and do not see a part number listed, please contact SPP-Support@agilent.com to discuss custom options. | [picture]

Bond Elut Plexa
Description | Unit | Part No.
Straight Barrel Cartridges
30 mg, 1 mL | 100/pk | 12109301
30 mg, 1 mL, Tabless | 100/pk | 12109301T
30 mg, 3 mL | 50/pk | 12109303
60 mg, 1 mL | 100/pk | 12109601
60 mg, 3 mL | 50/pk | 12109603
200 mg, 3 mL | 50/pk | 12109610
200 mg, 6 mL | 30/pk | 12109206
500 mg, 3 mL | 30/pk | 12109703
500 mg, 6 mL | 30/pk | 12259506
Bond Elut Jr
200 mg | 50/pk | 12169610B
Mega Bond Elut Plexa
500 mg, 12 mL | 20/pk | 327832
Other Formats
Bond Elut Plexa Prospekt cartridge, 2 mm | 96/pk | 12221305
Bond Elut Plexa 800 Series cartridge | 96/pk | 12281305
60 mg, 3 mL, Gerstel format | 50/pk | 167816G
200 mg, 3 mL, Gerstel format | 50/pk | 167822G

Bond Elut Plexa 96-well Plates
Description | 10 mg | 30 mg
1 mL round-well plates | A4969010 | A4969030
2 mL square-well plates | A3969010 | A3969030

Bond Elut Plexa Method for Polyaromatic Hydrocarbons

Twenty-four PAHs in drinking water by automated SPE with fast HPLC-FLD/UV detection (Pub No. 5990-7686EN)

Method

800 mL water sample + 5% isopropanol + internal standard (benzo[a]pyrene-d¹²)

Condition with 4 mL ethyl acetate + 4 mL dichloromethane + 4 mL methanol + 4 mL water

Load sample

Dry for 30 min

Elute with 4 mL ethyl acetate + 4 mL dichloromethane

Make up to 10 mL with ethyl acetate:dichloromethane (1:1)

Evaporate off 4 mL

Add 0.5 mL acetonitrile

HPLC/FLD chromatogram of a 5 μL injection of the 20 ppt PAH standard solution on the Agilent Pursuit 3 PAH column

Pursuit HPLC Columns

Bond Elut Plexa PCX

• Faster flow rates improve productivity

• Extraction cleanliness and reduced interference improve precision

• Simplified, single method for ease-of-use

Bond Elut Plexa PCX is another milestone in the development of simple and robust SPE methods. Plexa PCX uses a polymeric cation exchange resin that combines the outstanding properties of Bond Elut Plexa – superior flow characteristics and improved analytical performance – with strong cation exchange functionalities. This mixed-mode SPE sorbent removes neutral and acidic interferences from the matrix, concentrates basic analytes and therefore improves sensitivity in the determination of basic compounds.

The Plexa PCX particles are near mono-dispersed, resulting in homogenous packing. Reproducible results are the norm, with very good tube-to-tube and well-to-well performance. Ion suppression is reduced because the highly polar, hydroxylated polymer surface is entirely amide-free and does not provide binding sites for endogenous species such as proteins and lipids.

Plexa PCX comes with a simple, single method approach for basic drugs that offers improved recoveries, cleaner extracts and reduced method development time and cost. Flow rate is improved because Plexa PCX particles have much narrower particle size distribution with no fines to cause blockages.

  Typical Matrices

Plasma, urine, biological fluids and aqueous samples

  Primary Extraction Mechanism

Mixed mode: non-polar and cation exchange

Bond Elut Plexa PCX
Description | Unit | Part No.
Straight Barrel Cartridges
30 mg, 1 mL | 100/pk | 12108301
60 mg, 1 mL | 100/pk | 12108601
30 mg, 3 mL | 50/pk | 12108303
60 mg, 3 mL | 50/pk | 12108603
60 mg, 3 mL, Tabless | 50/pk | 12108603T
200 mg, 6 mL | 30/pk | 12108206
500 mg, 6 mL | 30/pk | 12258506
Other Formats
Bond Elut Plexa PCX Prospekt Cartridge, 2 mm | 96/pk | 12221306
Bond Elut Plexa PCX 800 Series Cartridge, 2 mm | 96/pk | 12281306
Gerstel format | 50/pk | 168016G

Bond Elut Plexa PCX 96-well Plates
Description | 10 mg | 30 mg
1 mL round-well plates | A4968010 | A4968030
2 mL square-well plates | A3968010 | A3968030

Typical Method for Bond Elut Plexa PCX

Sample:

100 μL plasma

Pretreatment:

Dilute 1:3 with 2% H₃PO₄

Conditioning:

1. 500 μL MeOH
2. 500 μL H₂O

Washes:

Acidic wash: | 500 μL aqueous 2% formic acid
Neutral wash: | 500 μL CH₃OH/CH₃CN (1:1, v/v)

Elution:

500 μL CH₃OH/CH₃CN + 5% NH₃ (28-30%)

Volumes stated are for Bond Elut 96 30 mg, 1 mL, P/N A4968030.

Bond Elut Plexa PAX

• Mixed mode, non-polar polymeric anion exchanger offers high level of analyte selectivity

• Exclusion of endogenous interferences offers superior cleanliness and minimizes ion suppression

• Simple, single method for ease-of-use, reduces method development time

Bond Elut Plexa PAX is a polymeric anion exchange product (PAX) that sets the performance standard in analyte cleanup and reproducibility for polar and non-polar acidic analytes. Existing polymeric anion exchange sorbents can exhibit a broad range of ion exchange capacity from batch to batch, leading to method irreproducibility and compromised data. Plexa PAX particles are functionalized using a proprietary process which allows anion exchange loadings to be controlled with a very high degree of reproducibility, giving more robust performance across the lifetime of your compound study or method.

This Plexa PAX polymeric mixed-mode SPE product comes with a simple, single method for non-polar acidic and polar acidic analytes that offers excellent clean up, even in complex matrices such as plasma. The optimized anion exchange methodology offers clean extracts, high recoveries and low RSDs, reducing method development time, sample repeats and overall cost per sample in the process.

Bond Elut Plexa PAX
Description | Unit | Part No.
Straight Barrel Cartridges
30 mg, 1 mL | 100/pk | 12107301
60 mg, 1 mL | 100/pk | 12107601
30 mg, 3 mL | 50/pk | 12107303
60 mg, 3 mL | 50/pk | 12107603
200 mg, 6 mL | 30/pk | 12107206
500 mg, 6 mL | 30/pk | 12257506

Bond Elut Plexa PAX 96-well Plates
Description | 10 mg | 30 mg
1 mL round-well plates | A4967010 | A4967030
2 mL square-well plates | A3967010 | A3967030

  Typical Matrices

Plasma, urine, biological fluids and aqueous samples

  Primary Extraction Mechanism

Mixed mode: non-polar and anion exchange

Typical Method for Bond Elut Plexa PAX

Sample:

100 μL human plasma

Pretreatment:

Dilute 1:3 with 2% NH₄OH

Conditioning:

1. 500 μL MeOH
2. 500 μL H₂O

Washes:

1. 500 μL H₂O
2. 500 μL MeOH

Elution:

500 μL 5% formic acid:MeOH

Volumes stated are for Bond Elut 96 1 mL Well Plate, P/N A4967010.

[picture] | TIPS & TOOLS View the core concepts of SPE and demonstrations of sample preparation, please visit www.agilent.com/chem/spevideo

Agilent Polymeric SPE

Reversed Phase Polymeric SPE

Bond Elut PPL

• Modified styrene-divinylbenzene polymer

• Large particle size allows fast extraction speeds

• High surface area and capacity for polar analytes

Bond Elut PPL is a styrene-divinylbenzene (SDVB) polymer that is modified with a proprietary non-polar surface. PPL will retain even the most polar classes of analytes, including phenols. The large particle size allows ease of flow for viscous or particulate-rich water samples, while the high surface area and strong hydrophobicity ensure reproducible extractions with high recoveries upon elution.

Bond Elut PPL is suitable for methods such as the US EPA Method 528, ‘Determination of Phenols in Drinking Water by SPE and Capillary GC/MS.’

Bond Elut PPL
Description | Unit | Part No.
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12105002
100 mg, 1 mL | 100/pk | 12105003
100 mg, 3 mL | 50/pk | 12105004
200 mg, 3 mL | 50/pk | 12105005
500 mg, 3 mL | 50/pk | 12105006
500 mg, 6 mL | 30/pk | 12255001
1 g, 3 mL | 50/pk | 12102148
1 g, 6 mL | 30/pk | 12255002
5 g, 60 mL | 16/pk | 12256087

  Typical Matrices

Water sources, biological fluids

  Primary Extraction Mechanism

Non-polar, electrostatic

Bond Elut ENV

• Modified styrene-divinylbenzene polymer

• Large particle size allows fast extraction speeds

• High surface area and capacity for polar analytes

Bond Elut ENV, a PS/DVB polymer, is designed for the extraction of polar organic residues. It contains 125 μm spherical particles, advantageous for high volume, fast flow-through applications.

Bond Elut ENV
Description | Unit | Part No.
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12105012
100 mg, 1 mL | 100/pk | 12105013
100 mg, 3 mL | 50/pk | 12105014
200 mg, 3 mL | 50/pk | 12105015
200 mg, 6 mL | 30/pk | 12255014
500 mg, 3 mL | 50/pk | 12105016
500 mg, 6 mL | 30/pk | 12255011
1 g, 6 mL | 30/pk | 12255012

  Typical Matrices

Water sources

  Primary Extraction Mechanism

Non-polar

Bond Elut LMS

• Ultra clean styrene-divinylbenzene polymer

• Optimized 75 μm particle size for reproducible flow

• High capacity and surface area for efficient extraction

Bond Elut LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or solvent mixtures of low ionic strength compatible with the HPLC mobile phase. These characteristics allow easy compatibility with LC/MS or other delicate analytical techniques.

Bond Elut LMS
Description | Unit | Part No.
Straight Barrel Cartridges
25 mg, 1 mL | 100/pk | 12105021
100 mg, 1 mL | 100/pk | 12105023
100 mg, 3 mL | 50/pk | 12105024
200 mg, 3 mL | 50/pk | 12105025
500 mg, 3 mL | 50/pk | 12105026
500 mg, 6 mL | 30/pk | 12255021
1 g, 6 mL | 30/pk | 12255022

Bond Elut LMS 96-well Plates
Description | 10 mg | 25 mg
1 mL round-well plates | A4961010 |
2 mL square-well plates | A3961010 | A3961025

  Typical Matrices

Urine, plasma, biological fluids

  Primary Extraction Mechanism

Non-polar

Mixed Mode Polymeric SPE

Bond Elut NEXUS and Bond Elut NEXUS WCX

• Large particle size allows excellent flow for viscous samples

• Non-conditioning method saves time and improves throughput

• WCX offers enhanced selectivity for certain analytes such as quaternary amine drugs

Bond Elut NEXUS is an ultra-clean polymeric sorbent which has bi-modal porosity and a high surface area. NEXUS offers a non-polar retention mechanism with no pre-conditioning required. The large particle size makes NEXUS ideal for extractions from highly viscous samples such as horse urine.

Based on the same base polymer technology, Bond Elut NEXUS WCX is a weak cation exchange sorbent that offers extra selectivity for analytes such as quaternary ammonium drugs and anabolic steroids.

  Typical Matrices

Horse urine, urine, biological fluids

  Primary Extraction Mechanism

Non-polar

Bond Elut NEXUS and Bond Elut NEXUS WCX
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
30 mg, 10 mL | 50/pk | 12113100
60 mg, 10 mL | 50/pk | 12113101
Straight Barrel Cartridges
30 mg, 1 mL | 100/pk | 12103100
60 mg, 3 mL | 100/pk | 12103101
60 mg, 3 mL, NEXUS WCX | 100/pk | 12102157
200 mg, 6 mL | 30/pk | 12103102
200 mg, 12 mL | 20/pk | 12253101
500 mg, 12 mL | 20/pk | 12253102
500 mg, 20 mL | 20/pk | 12253103

Bond Elut NEXUS 96-well Plates
Description | 30 mg | 60 mg
1 mL round-well plates | A4962030 |
2 mL square-well plates |  | A3962060

References

Wynne, PM, Barry, DC, Vine, JH & Simpson, NKJ (2004) Approaches to the solid phase extraction of equine urine. Chromatography, 59, S51-S60.

Wynne, PM, Barry, DC, Vine, JH & Simpson, NKJ (2000) An improved method for the extraction of anabolic steroids from equine urine. In: RB Williams, E Houghton & J Wade (eds) Proc. 13th Int. Conf. Racing Analysts and Veterinarians. R & W Publications, Newmarket, UK.

Silica-Based SPE

Reversed Phase (Non-Polar) Silica SPE

Reversed phase sorbents are non-polar and are used to retain (extract) non-polar analytes from polar matrices. For reversed phase sorbents, retention decreases as the eluting solvent becomes more non-polar.

Bond Elut C18

• The most hydrophobic, bonded silica sorbent

• Extremely retentive for non-polar compounds

• Effective for desalting aqueous mixtures

Bond Elut C18 is the most hydrophobic, bonded silica sorbent in the Bond Elut range. It is the most popular SPE sorbent because of its extremely retentive nature for non-polar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most organic analytes from aqueous matrices. When analyzing small to intermediate molecules, Bond Elut C18 can be used for desalting aqueous matrices prior to ion exchange, as salts pass through the sorbent unretained.

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar

TIPS & TOOLS |
Tabless (flangeless) cartridges are suitable for use with many automated SPE systems. Tabless products are typically designated with a “T” in the part number. If you need a tabless cartridge and do not see a part number listed, please contact SPP-Support@agilent.com to discuss custom options. | [picture]

Bond Elut C18 Flash cartridges, 12256060

*Tubes need to be inserted into a VersaPlate base plate, P/N 75400000

Preassembled 96-well plate, 75401050

VersaPlate tubes, 75501050

Bond Elut C18 EWP

• No exclusion of large molecules

• Good for desalting proteins

• Successful separation of proteins, peptides or nucleotides

Bond Elut C18 EWP is based on standard particle size silica but with 500Å pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.

Bond Elut C18 EWP
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
50 mg, 10 mL | 50/pk | 12113068
500 mg, 10 mL | 50/pk | 12113071
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102136
100 mg, 1 mL | 100/pk | 12102137
500 mg, 3 mL | 50/pk | 12102139
1 g, 6 mL | 30/pk | 12256130

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar

Bond Elut C18 OH

• Silanol activity permits metabolite fractionation

• Tight QC tolerances deliver batch-to-batch reproducibility

• 150Å pore size expands utility to higher molecular weight compounds

Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18.

Bond Elut C18 OH
Description | Unit | Part No.
Straight Barrel Cartridges
100 mg, 1 mL | 100/pk | 12102020
500 mg, 3 mL | 50/pk | 12102046
1 g, 6 mL | 30/pk | 12256040

  Typical Matrices

Aqueous samples, biological fluids, non-polar extracts

  Primary Extraction Mechanism

Non-polar, hydrogen bonding

Bond Elut C8

• Excellent for strongly-retained analytes

• Polar interactions not significant

• Less retentive than C18

Bond Elut C8 is very similar in properties to C18, but is not as retentive for non-polar compounds, due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution. The potential for polar interactions is somewhat higher than for C18 because there is less coverage of the silica surface. These polar interactions are not, however, a significant property of C8.

Bond Elut C8
Description | Unit | Part No.
Bond Elut Jr
500 mg | 100/pk | 12162029B
1 g | 100/pk | 12166002B
Large Reservoir Capacity (LRC) Cartridges
100 mg, 10 mL | 50/pk | 12113075
200 mg, 10 mL | 50/pk | 12113025
500 mg, 10 mL | 50/pk | 12113028
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102059
50 mg, 3 mL | 50/pk | 12105028
100 mg, 1 mL | 100/pk | 12102002
100 mg, 1 mL | 500/pk | 52102002
100 mg, 3 mL | 50/pk | 12102100
200 mg, 3 mL | 50/pk | 12102026
200 mg, 3 mL | 500/pk | 52102026
500 mg, 3 mL | 50/pk | 12102029
500 mg, 6 mL | 30/pk | 12102053
1 g, 6 mL | 30/pk | 12256002
5 g, 20 mL | 20/pk | 12256024
10 g, 60 mL | 16/pk | 12256032
Other Formats
Prospekt cartridge, 800 Series, 2 mm | 96/pk | 12281002
100 mg, 3 mL, Gerstel format | 50/pk | 161618G
200 mg, 3 mL, Gerstel format | 50/pk | 161622G
500 mg, 3 mL, Gerstel format | 50/pk | 161632G

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar

*Tubes need to be inserted into a VersaPlate base plate, P/N 75400000

Bond Elut PH

• Added selectivity compared to other non-polar sorbents

• Enhanced retention of planar, conjugated organic molecules

• Similar polarity to C8

Bond Elut PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalized phases such as C8 or cyclohexyl. The electron density present in the aromatic ring enhances retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.

  Typical Matrices

Aqueous and biological fluids

  Primary Extraction Mechanism

Non-polar

Bond Elut CH (cyclohexyl)

• Non-polar CH with polarity similar to C2

• Retains polar analytes from aqueous matrices

• Good choice when common non-polar sorbents do not provide the required selectivity

Bond Elut CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C2 sorbent. Bond Elut CH is often a good choice when non-polar sorbents such as C18, C8, or C2 do not provide the desired selectivity.

Bond Elut CH (cyclohexyl)
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
500 mg, 10 mL | 50/pk | 12113032
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102063
100 mg, 1 mL | 100/pk | 12102006
500 mg, 3 mL | 50/pk | 12102033
1 g, 6 mL | 30/pk | 12256005
2 g, 12 mL | 20/pk | 12256039

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar

Bond Elut C1

• Least retentive of all alkyl group bonded phases

• Easy retention and release of polar compounds

• Easy retention and release of multi-functional compounds

Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for non-polar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention and elution of polar and multi-functional analytes can still be achieved.

Bond Elut C1
Description | Unit | Part No.
Straight Barrel Cartridges
100 mg, 1 mL | 100/pk | 12102004
100 mg, 3 mL | 50/pk | 12102090
500 mg, 3 mL | 50/pk | 12102031

  Typical Matrices

Urine, plasma, biological fluids

  Primary Extraction Mechanism

Non-polar, polar (as a normal phase extraction)

Bond Elut C2

• Low carbon load sorbent

• Can be used alongside CN and C8 phases

• Popular for drug extraction from plasma and for flat baselines

Bond Elut C2 is a fairly non-polar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.

Bond Elut C2
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
100 mg, 10 mL | 50/pk | 12113003
500 mg, 10 mL | 50/pk | 12113029
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102060
50 mg, 3 mL | 50/pk | 12105029
100 mg, 1 mL | 100/pk | 12102003
100 mg, 3 mL | 50/pk | 12102117
200 mg, 3 mL | 50/pk | 12102027
500 mg, 3 mL | 50/pk | 12102030
500 mg, 6 mL | 30/pk | 12102115
1 g, 6 mL | 30/pk | 12256003

Bond Elut C2 96-well Plates
Description | 50 mg | 100 mg
1 mL round-well plates | A4961150 | A496111C

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar

Normal Phase (Polar) Silica SPE

Normal phase sorbents are polar and used to retain (extract) polar analytes. For normal phase sorbents, retention decreases as the eluting solvent becomes more polar.

Bond Elut SI

• Highly polar phase retains polar molecules from non-polar matrices

• High purity silica

• Separate compounds with very similar structures

Native silica is generally regarded as the most polar SPE sorbent available. Bond Elut SI is particularly effective at separating compounds with a very similar structure. Applying the analytes in a non-polar solvent, then increasing the solvent polarity by increasing the concentration of a polar modifier, such as THF or ethyl acetate, delivers effective separations.

  Typical Matrices

Non-polar organics, oils, lipids

  Primary Extraction Mechanism

Polar

Bond Elut CN-E

• Ideal for extracting aqueous analytes

• Retention in aqueous and organic matrices

• Useful for many applications

A medium polarity sorbent with many uses, Bond Elut CN-E is ideal for applications in which extremely non-polar compounds would be irreversibly retained on high carbon load sorbents such as C8 and C18. This endcapped version of the cyano sorbent is best utilized when extracting analytes from an aqueous matrix.

Bond Elut CN-E
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
500 mg, 10 mL | 50/pk | 12113033
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102064
100 mg, 1 mL | 100/pk | 12102007
500 mg, 3 mL | 50/pk | 12102034

References

Pucci, V, Bugamelli, F, Mandrioli, R, Bartoletti, C, Rossi, N & Raggi, MA (2003) Liquid chromatographic analysis of the cis(Z)- and trans(E)-isomers of clopenthixol in human plasma using a novel solid phase extraction procedure. J. Chromatogr. B., 792, 313-321.

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Non-polar, dipole

Bond Elut Diol (2OH)

• Provides polar and non-polar modes

• Strong hydrogen bonding with analytes

• Resembles un-bonded silica in its capabilities

Bond Elut Diol resembles un-bonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

Bond Elut Diol (2OH)
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges
100 mg, 10 mL | 50/pk | 12113009
500 mg, 10 mL | 50/pk | 12113035
Straight Barrel Cartridges
50 mg, 1 mL | 100/pk | 12102067
100 mg, 1 mL | 100/pk | 12102009
500 mg, 3 mL | 50/pk | 12102036
1 g, 6 mL | 30/pk | 12256007

  Typical Matrices

Aqueous, biological fluids, non-polar organics

  Primary Extraction Mechanism

Polar and non-polar

Bond Elut NH2

• Normal phase or anion exchange sorbent

• Weaker anion exchange than SAX

• Amenable to separating structural isomers

Bond Elut NH2 is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged) and is therefore a better choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol and SI sorbents, Bond Elut NH2 is excellent for the separation of structural isomers.

References

Schenck, F, Lehotay, S, & Vega, V (2002) Comparison of solid phase extraction sorbents for cleanup of pesticide residue analysis in fresh fruit and vegetables. J. Sep. Sci., 25, 883-890.

  Typical Matrices

Aqueous samples, biological fluids, buffered organics

  Primary Extraction Mechanism

Weak anion exchange

The isolation of lipids from serum and tissue

Extraction Method

Matrix: | Sorbent Conditioning: | Apply Sample:
Chloroform extract of serum or adipose tissue | Hexane | Through Bond Elut NH2 cartridge

Elution 1:

(Neutral lipids)

(All except fatty acids and phospholipids) – 2:1 chloroform: 2-propanol

(Fatty acids)

2% acetic acid in diethyl ether

(Phospholipids)

Methanol

The neutral lipid fraction is then dried down, reconstituted in hexane, and passed through a second NH2 tube conditioned with hexane.

Elution 2:

(Cholesterol esters)

Hexane

Another Bond Elut NH2 sorbent column is attached below the existing one to trap cholesterol that breaks through the first during triglyceride elution.

Elution 3:

(Triglycerides)

Hexane containing 1% diethyl ether and 10% methylene chloride

The Bond Elut NH2 tubes are separated, cholesterol is eluted from both, and finally the di- and monoglycerides are eluted from the upper NH2 tube.

Elution 4:

(Cholesterol)

5% ethyl acetate in hexane

(Diglycerides)

15% ethyl acetate in hexane

(Monoglycerides)

2:1 chloroform:methanol

Simpson, N & Van Horne, C (eds) (1993) The Handbook of Sorbent Extraction Technology. Varian, Inc., Walnut Creek CA, USA.

Ion Exchange Silica SPE

Ion exchange phases are more dependent on pH, ionic strength, and counter-ion strength than on solvent strength. These phases depend on ionic interactions as the primary retention mechanism.

Bond Elut SAX

• Retains compounds that elute from weak anion exchange sorbents

• Selectivity can be user-modified for increased flexibility

• Minimal non-polar interactions

Bond Elut SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds such as carboxylic acids, which may not retain effectively on weak anion exchange sorbents.

  Typical Matrices

Aqueous samples, biological fluids, buffered organics

  Primary Extraction Mechanism

Anion exchange

Bond Elut SAX VersaPlate Formats
Description | Particle Size (μm) | 50 mg
Preassembled 96-well plate | 40 | 75408050
VersaPlate tubes, 96/pk* | 40 | 75508050

*Tubes need to be inserted into a VersaPlate base plate, P/N 75400000

Bond Elut SCX

• Useful for compounds with both cationic and non-polar characteristics

• Superior cleanup from a single sorbent

• Very low pKa ligand elicits strong analyte interaction

Bond Elut SCX is a strong cation exchanger with a very low pKa. Although the pKa is similar to Bond Elut PRS, the presence of the benzene ring in the functional group increases the potential for non-polar interactions. This non-polar characteristic becomes particularly important when conducting ion exchange from aqueous systems, where selectivity towards compounds exhibiting cationic and non-polar character is seen.

  Typical Matrices

Aqueous samples, biological fluids, buffered organics

  Primary Extraction Mechanism

Cation exchange

References

Codony, R, Compañó, R, Granados, M, Garcia-Regueiro, JA & Dolors Prat, M (2002) Residue analysis of macrolides in poultry muscle by liquid chromatography-electrospray mass spectrometry. J. Chromatogr. A, 959, 131-141.

Horie, M, Saito, K, Ishii, R, Yoshida, T, Haramaki, Y & Nakazawa, H (1998) Simultaneous determination of five macrolide antibiotics in meat by high performance liquid chromatography. J. Chromatogr. A, 812, 295-302.

Stubbings, G, Tarbin, J, Cooper, A, Shaman, M. Bigwood, T & Robb, P (2005) A multi-residue cation-exchange clean up procedure for basic drugs in produce of animal origin. Analyt. Chim. Acta, 547, 262-268.

Bond Elut PRS

• Strong cation exchange sorbent, also capable of polar and hydrogen bonding interactions

• No appreciable non-polar interactions

• Unique selectivity properties

Bond Elut PRS is a strong cation exchange sorbent that is also relatively high in polarity. With no appreciable degree of hydrophobicity in non-polar solvents, PRS is capable of polar and hydrogen bonding interactions. Due to the very low pKa of PRS, it is recommended for weaker cationic species such as pyridinium compounds.

  Typical Matrices

Aqueous, biological fluids, buffered organics

  Primary Extraction Mechanism

Cation exchange

Bond Elut PRS
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
100 mg, 10 mL | 50/pk | 12113012
500 mg, 10 mL | 50/pk | 12113038
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102074
100 mg, 1 mL | 100/pk | 12102012
200 mg, 3 mL | 50/pk | 12102094
500 mg, 3 mL | 50/pk | 12102039
1 g, 6 mL | 30/pk | 12256010

Bond Elut PSA

• Alternative choice to Bond Elut NH2 for polar compounds

• Higher ionic capacity than NH2

Bond Elut PSA is an alkylated amine sorbent that contains two different amino functionalities – one secondary and one primary. This gives a slightly higher pKa and ionic capacity compared to Bond Elut NH2. PSA has a significantly higher carbon load than most amino functional sorbents, thus is a better choice for polar compounds, which retain too strongly on Bond Elut NH2.

  Typical Matrices

Aqueous samples, biological fluids, buffered organics

  Primary Extraction Mechanism

Weak anion exchange

Bond Elut PSA
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
500 mg, 10 mL | 50/pk | 12113041
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102077
100 mg, 1 mL | 100/pk | 12102015
500 mg, 3 mL | 50/pk | 12102042
1 g, 6 mL | 30/pk | 12256140
2 g, 12 mL | 20/pk | 12256055
Bond Elut Jr |  |
500 mg | 100/pk | 12162042B
1 g | 100/pk | 12166050B

Bond Elut CBA

• Cation exchange with no need for extreme basic conditions

• Wider selectivity range provides more eluent options

• Polar or non-polar depending on matrix or solvent

CBA is a mid-polarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider range of counter-ions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.

  Typical Matrices

Aqueous samples, biological fluids

  Primary Extraction Mechanism

Weak cation exchange

Bond Elut CBA
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
100 mg, 10 mL | 50/pk | 12113011
500 mg, 10 mL | 50/pk | 12113037
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102073
100 mg, 1 mL | 100/pk | 12102011
100 mg, 3 mL | 50/pk | 12102097
200 mg, 3 mL | 50/pk | 12102124
500 mg, 3 mL | 50/pk | 12102038
1 g, 6 mL | 30/pk | 12256009
2 g, 12 mL | 20/pk | 12256058

References

Murayama, N. & Sudo, K (1997) High performance liquid chromatographic method for determination of DX-9065a, a novel anticoagulant, in human urine and feces using cation-exchange solid-phase extraction. J. Chromatogr. Biomed. Appl., 692, 389-396.

Bond Elut DEA

• Weak anion exchanger

• More polar than C8 but less polar than C2 or CN

• Alkyl side chains confer moderately non-polar characteristics

Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties but with a slightly lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C8 but less polar than C2 or CN-E.

  Typical Matrices

Water, biological fluids, non-polar extracts

  Primary Extraction Mechanism

Weak anion exchange

References

Kline, W., Matuszewski, B & Bayne, W (1990) Determination of 4-amino-1-hydroxybutane-1,1-bisphosphonic acid in urine by automated pre-column derivatization with 2,3-naphthalene dicarboxyaldehyde and high performance liquid chromatography with fluorescence detection. J. Chromatogr. Biomed.l Appl., 534, 139-149.

Mixed Mode Silica SPE

Bond Elut AccuCAT

• SCX and SAX functionalities offer broad analyte extraction potential

• Ultra clean, mixed sorbent bed delivers reproducible extractions

• Compatible with many biological fluids for easy method transfer

Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from bio-fluids.

  Typical Matrices

Urine, plasma and biological fluids, beverages and food

  Primary Extraction Mechanism

Strong cation and anion exchange

Bond Elut AccuCAT
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
200 mg, 10 mL | 60/pk | 12282005
600 mg, 10 mL | 60/pk | 12282001
Straight Barrel Cartridges |  |
200 mg, 3 mL | 60/pk | 12282003
200 mg, 6 mL | 30/pk | 12282004
400 mg, 6 mL | 30/pk | 12282006
600 mg, 3 mL | 60/pk | 12282002

References

Andrzejewski, D, Roach, JAG, Gay, ML and Musser, SM (2004) Analysis of coffee for the presence of acrylamide by LC-MS/MS. J. Agric. Food Chem., 52, 1996-2002.

Lenders, JW, Eisenhofer, G, Armando, I, Keiser, HR, Goldstein, DS and Kopin, IJ (1993) Determination of metanephrines in plasma by liquid chromatography with electrochemical detection. Clin. Chem., 39, 97-103.

Bond Elut Certify

• Special mixed-mode sorbent bed

• Broad application range for aqueous extraction

• Bimodal, non-polar and strong cation exchange

The Bond Elut Certify extraction cartridge is a mixed mode sorbent containing non-polar and C8 strong cation exchanger functionalities. Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for the extraction of a wide range of compounds from a diverse range of aqueous matrices. Rely on the Certify products for consistent performance and availability in a range of formats to support automation and high sample throughput.

Bond Elut Certify VersaPlate cartridges

  Typical Matrices

Urine, plasma, saliva, blood, biological fluids

  Primary Extraction Mechanism

Non-polar and strong cation exchange

Bond Elut Certify II

• Ideal for non-polar and anionic compounds

• Optimized for acidic drug analysis

• Bimodal, non-polar and strong anion exchange

Bond Elut Certify II is designed for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices for forensic use. Certify II is a mixed-mode cartridge with non-polar C8 and strong anion exchange (SAX) functionalities. It has been optimized for acidic drugs such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen and other compounds that possess both non-polar and anionic characteristics.

  Typical Matrices

Urine, plasma, saliva, blood, biological fluids

  Primary Extraction Mechanism

Non-polar and strong anion exchange

Inorganic SPE

The following SPE phases have varying degrees of polarity and surface acidity or basicity. They are primarily used to retain polar analytes. For these phases, solvent retention generally decreases as the solvent becomes more polar.

Bond Elut Florisil

• Pesticide Residue (PR) grade

• For cleanup of polar interferences from non-polar samples

• Economical

• Fast flow, ideal for viscous samples

Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for the isolation of polar compounds from non-polar matrices. The larger particle size of the sorbent enables fast flow for large sample volumes and is therefore an attractive alternative to silica if the sample matrix is particularly viscous.

  Typical Matrices

Non-polar organics

  Primary Extraction Mechanism

Polar compounds

Bond Elut Florisil
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
500 mg, 10 mL | 50/pk | 12113049
Straight Barrel Cartridges |  |
100 mg, 1 mL | 100/pk | 12102024
200 mg, 3 mL | 50/pk | 12102129
500 mg, 6 mL | 30/PK | 12102159
500 mg, 3 mL | 50/pk | 12102050
1 g, 3 mL | 50/pk | 12102109
1 g, 6 mL | 30/pk | 12256014
1 g, 6 mL | 250/pk | 52256014
1 g, 20 mL | 20/pk | 12256047
2 g, 12 mL | 20/pk | 12256022
2 g, 20 mL | 20/pk | 12256046
5 g, 20 mL | 20/pk | 12256030
10 g, 60 mL | 16/pk | 12256038
Bond Elut Jr |  |
500 mg | 100/pk | 12162050B
1 g | 100/pk | 12166014B
Other Formats |  |
500 mg, 3 mL, Gerstel format | 50/pk | 164632G

Bond Elut Alumina

• Available in acidic (A), basic (B) and neutral (N) formats

• High extraction efficiency

• Better high pH stability than unfunctionalized silica

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The small particle size of the Bond Elut Alumina range ensures high extraction efficiency even when small bed masses are used.

  Typical Matrices

Non-polar organics

  Primary Extraction Mechanism

Polar

Bond Elut Alumina A
Description | Unit | Part No.
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102069
500 mg, 3 mL | 50/pk | 12102047
1 g, 6 mL | 30/pk | 12256043
Bond Elut Jr |  |
1 g | 100/pk | 12166043B

Bond Elut Alumina B
Description | Unit | Part No.
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102070
500 mg, 3 mL | 50/pk | 12102048
1 g, 6 mL | 30/pk | 12256044
Bond Elut Jr |  |
500 mg | 100/pk | 12162048B
1 g | 100/pk | 12166044B

Bond Elut Alumina N
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
500 mg | 50/pk | 12113048
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 12102071
100 mg, 1 mL | 100/pk | 12102023
500 mg, 3 mL | 50/pk | 12102049
500 mg, 6 mL | 1000/pk | 221032B
1 g, 6 mL | 30/pk | 12256086
20 g, 60 mL | 16/pk | 12256059
Bond Elut Jr |  |
500 mg | 100/pk | 12162049B
1 g | 100/pk | 12166045B

Bond Elut Sodium Sulfate Drying Cartridges

• Highly effective pre-packed dessicant

• Clean ACS grade, anhydrous sodium sulfate

• Pre-packed for convenience

Simplify sodium sulfate mediated drying steps by using cartridges pre-packed with ACS grade, granular anhydrous sodium sulfate. Available in three formats (LRC, Bond Elut Jr and straight barrels).

Bond Elut Jr cartridges have top and bottom luer fittings, allowing for easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Bond Elut Sodium Sulfate Drying Cartridges
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
1 g, 10 mL | 100/pk | 12131033
Straight Barrel Cartridges |  |
15 g, 60 mL | 100/pk | 12132004
Bond Elut Jr |  |
1.4 g | 100/pk | 12162052B
2.2 g | 100/pk | 12162054B
3 g | 100/pk | 12162051B

[picture] | TIPS & TOOLS Agilent offers Bond Elut Adapters compatible with these tube formats. View Here.

Mega Bond Elut Flash

• Convenient disposable cartridges eliminate the need for packing glass columns

• Flexible “open” tube design for either liquid or solid samples

• Reliable, consistent flow characteristics deliver high-resolution performance

Mega Bond Elut Flash cartridges offer excellent levels of performance and productivity for the purification of organic compounds, and also for scale-up, solid phase extraction. Pre-packed, disposable cartridges offer greater convenience than glass columns that require washing, drying and re-packing after every sample.

Bond Elut C18 Flash cartridges, 12256060

Specialty SPE

Bond Elut Carbon

• Excellent retention for small organics, including those that are too polar to retain on C18 or polymeric SPE

• Removal of chlorophyll and other pigments leads to fewer chromatographic or mass interferences

• Broader retention and easier elution of analytes across the polarity range, for improved multi-residue analysis

Bond Elut Carbon cartridges are packed with ultra-pure graphitized carbon particles that have been optimized for the absorption of pigments in food, fruits and vegetables, and small organic residues in waste water. The powerful retention mechanisms of these products are appropriate for a broad range of analytes. In addition, careful manufacturing techniques result in lower carbon fines on the wall of the device.

  Typical Matrices

Organic plant and tissue extracts

  Primary Extraction Mechanism

Wide range non-polar retention

Bond Elut Carbon
Description | Unit | Part No.
Straight Barrel Cartridges |  |
50 mg, 1 mL | 100/pk | 126414
100 mg, 1 mL | 100/pk | 126418
250 mg, 6 mL | 30/pk | 12102201
500 mg, 6 mL | 30/pk | 12252201
Bond Elut Jr |  |
250 mg | 100/pk | 446424
400 mg | 100/pk | 466430

[picture] | GLOBAL TIP The Japanese Positive List System for Agriculture Residues in Food can be found at http://www.ffcr.or.jp

Bond Elut Carbon/NH2
Description | Unit | Part No.
Straight Barrel Cartridges |  |
300/500 mg, 6 mL | 30/pk | 2264265032
500/500 mg, 6 mL | 30/pk | 12252202
500/500 mg, 20 mL | 20/pk | 3664325032

Bond Elut Carbon/PSA
Description | Unit | Part No.
Straight Barrel Cartridges |  |
250/250 mg, 3 mL | 50/pk | 12102042C250
500/500 mg, 6 mL | 30/pk | 12102042C500

Method for the simultaneous monitoring of pesticide residues in agricultural products – extraction, refining (cleanup) and quantitative analysis

Bond Elut Cellulose

• High purity micro-granular cellulose with high a-cellulose content

• Stable across a broad pH range

• Extremely low metal content (Fe, Cu <5 ppm)

Bond Elut Cellulose columns use a pure micro-granular cellulose powder that is packed between two 20 μm polypropylene frits. The cellulose phase is very stable over a wide pH range with extremely low metal content. The combination of surface area and polymeric structure results in a sorbent with excellent capacity. The cellulose media contains numerous hydroxyl groups; because of it polar nature, it is able to accept high loading of many polar substances from aqueous and organic phases.

  Typical Matrices

Aqueous samples and non-polar organics

  Primary Extraction Mechanism

Polar (Hydroxyl)

Bond Elut Cellulose
Description | Unit | Part No.
Straight Barrel Cartridges |  |
300 g, 3 mL | 500/pk | 12102095

Bond Elut PCB

• Optimized bed mass affords excellent extraction reproducibility

• Special dual-phase enhances PCB selectivity

• All extractions can be completed with one solvent to simplify procedures

Bond Elut PCB is a specially designed sorbent which allows for the easy extraction of polychlorinated biphenyl (PCB) compounds from a variety of matrices. Desired analytes can be loaded and eluted using a simple, single solvent method prior to analysis by GC/ECD.

  Typical Matrices

Water sources

  Primary Extraction Mechanism

Polar

Bond Elut PCB
Description | Unit | Part No.
Straight Barrel Cartridges |  |
1 g, 3 mL | 50/pk | 12105032

Bond Elut Mycotoxin

• Simple methodology saves time and increases throughput

• Use with a broad range of food matrices

• Economic and time-saving alternative to immunoaffinity techniques

Bond Elut Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenone analysis by LC/MS/MS. Results are comparable or superior to competing methods, including immunoaffinity columns (IAC) and charcoal/alumina columns. The sorbent is a proprietary silica-based ion exchange material.

The Bond Elut Mycotoxin method for extraction and cleanup is successful with a variety of food and grain sample types, including wheat, corn, durum, oats, bread, muesli and infant food.

Bond Elut Mycotoxin is easy to use and acts in a selective non-retention way – the toxin analytes pass through the cartridge while the food matrix components are retained.

  Typical Matrices

Aqueous samples and polar organic grain extracts (beer, wine, sake), grains, and other foods

  Primary Extraction Mechanism

Ionic cleanup

Bond Elut Mycotoxin
Description | Unit | Part No.
Straight Barrel Cartridges |  |
500 mg, 3 mL | 50/pk | 12102167
Bond Elut Jr |  |
500 mg | 100/pk | 12165001B

References

Kiötzel, M, Lauber, U & Humpf, H-U (2006) A new solid phase extraction clean-up method for the determination of 12 type A and B trichothecenes in cereals and cereal-based food by LC-MS/MS. Mol. Nutr. Food Res, 50, 261-269.

Bretz, M, Beyer, M, Cramer, B & Humpf, H-U (2006) Stable isotope dilution analysis of the fusarium mycotoxins deoxynivalenol and 3-acetyldeoxynivalenol. Mol. Nutr. Food Res., 50, 251-260.

General Mycotoxin Methods

For Solids

1. Finely grind 25 g sample and extract with a solution of 100 mL acetonitrile/water (80:20) by blending at high speed for 3 min. For simultaneous determination of zearalenone, spike extract at a level of 50 ng/g sample with zearalanone (ZAN) solution in acetonitrile internal standard. Filter.

2. Pass 4 mL of the filtrate through a Bond Elut Mycotoxin column.

3. Evaporate 2 mL of eluate to dryness at 50 °C under a gentle stream of nitrogen.

4. Reconstitute in 0.5 mL ACN/H₂O (1:4; v/v).

5. Inject 10 μL into LC for analysis.

For Beverages

1. Sonicate the beverage sample for 30 min. Filter.

2. Pass 4 mL of the filtrated sample extract through a Bond Elut Mycotoxin cartridge.

3. Evaporate 2 mL of the eluate to dryness at 50 °C under a gentle stream of nitrogen.

4. Reconstitute in 0.5 mL ACN/H₂O (20/80; v/v).

5. Inject into LC/MS QQQ.

This application shows the optimized extraction and cleanup of type A- and B-trichothecenes [deoxynivalenol [DON], HT-2 toxin [HT-2], T-2 toxin [T-2] and zearalenone (ZEA).

Bond Elut PBA

• Unique phenylboronic acid sorbent

• High specificity for cis-diol compounds

• Amenable to a broad range of bio-molecule applications

Bond Elut PBA is a unique silica SPE sorbent containing a phenylboronic acid functionality that can retain analytes via a reversible covalent bond. This very strong covalent retention mechanism enables high specificity and cleanliness. The boronate group has a strong affinity for cis-diol containing compounds such as catechols, nucleic acids, some proteins, carbohydrates and PEG compounds. Aminoalcohols, alpha-hydroxy amides, keto compounds, and others can also be retained.

  Typical Matrices

Plasma, urine, aqueous samples and biological fluids

  Primary Extraction Mechanism

Covalent bonding

Bond Elut PBA
Description | Unit | Part No.
Large Reservoir Capacity (LRC) Cartridges |  |
100 mg, 10 mL | 50/pk | 12113018
Straight Barrel Cartridges |  |
100 mg, 1 mL | 20/pk | 12102018
100 mg, 1 mL | 100/pk | 12102019
100 mg, 3 mL | 50/pk | 12102127
500 mg, 6 mL | 30/pk | 12102105

Bond Elut PBA 96-well Plates
Description | 100 mg
1 mL round-well plates | A496121C
2 mL square-well plates | A396121C

Generic Method

Condition:

1. 70:30 H₂0:ACN with 1% TFA

2. 50 mM phosphate buffer (pH 10)

Sample Addition:

Sample should be buffered to pH 8.5 with 50 mM phosphate buffer

Interference Wash:

10 mM phosphate buffer (pH 8.5) with 5% ACN

Analyte Elution:

70:30 H₂O:ACN with 1% TFA (pH <5.0)

Compound Class | Examples
Polyhydroxy | Mannitol, fructose-6-phosphate, CDP-ethanol-amine, glycoproteins
Aromatic O-dihydroxy | Catechols, tannins, epinephrine
α-Hydroxy acids | Lactate, 6-phospho-gluconate
Aromatic O-hydroxy acids and amines | Salicylate, salicylamide
1,3-Dihydroxy | Tris, pyridoxine
Diketo & triketo | Dehydroascorbic acid, benzil, alloxan
Other dihydroxys | Steroids, prostaglandins

EnvirElut

• Extreme purity offers cleanliness in extract

• High capacity allows for the processing of large sample volumes

• Broad compound specificity

EnvirElut sorbents are specially designed for the extraction of a wide range of compounds from aqueous matrices. EnvirElut PAH and Pesticides are available in standard SPE straight barrel cartridges, which can be used on conventional vacuum manifolds such as the Vac Elut SPS 24.

  Typical Matrices

Water sources, extracted soil samples

  Primary Extraction Mechanism

Non-polar

EnvirElut
Description | Unit | Part No.
Straight Barrel Cartridges |  |
1 g, 3 mL (PAH) | 50/pk | 12272007
1 g, 6 mL (PAH) | 30/pk | 12272005
500 mg, 6 mL (Pesticide) | 30/pk | 12272004
5 g, 20 mL (Oil + Grease) | 20/pk | 12272001
US EPA 1664, 20 mL | 20/pk | 12272020
NH2/EnvirElut (100 mg/500 mg), 3 mL | 50/pk | 12102158
5 g, 20 mL (Phenols) | 20/pk | 12272002

Solid Phase Microextraction

Solid phase microextraction (SPME) is a technique for extracting analytes from solid, liquid or gaseous samples by adsorbing them onto the SPME fiber and then desorbing them into an inlet, either on a gas chromatograph (GC) or an HPLC system. SPME is amenable to automation using an autosampler or it can be performed manually as well. Agilent offers SPME fibers in a range of chemistries, formats, and for use with autosamplers or manual injections. Kits are also available to support method development, offering a variety of fiber types and configurations within a single kit.

Solid Phase Microextraction Fibers

When ordering SPME fibers, note that the fiber kits contain only the fibers. For a first-time order, you will also need to order the appropriate fiber holder for your needs. SPME fibers can be used multiple times depending on the application and when treated with the proper care and caution. Each fiber has a color-coded or notched hub indicating the type of coating on the fiber.

TIPS & TOOLS

The Merlin Microseal system can reduce septum coring and help eliminate septum bleed. Only use the Merlin Microseal with a 23 gauge SPME fiber assembly. To replace your GC septum nut with a Merlin microseal, you can find Merlin Microseal kits in the GC and GC/MS Columns & Supplies Catalog, publication number 5991-1058EN

Solid Phase Microextraction Kits

SPME Fiber kits contain three fibers. Note that the fiber coating thickness (df) is expressed in μm, and when multiple phase types are included in a kit, the fiber coatings are listed in the respective order that the phases are listed in the description.

[picture] | TIPS & TOOLS Agilent offers inlet liners designed to work with SPME applications for best performance. These liners can be found in the GC and GC/MS Columns & Supplies Catalog, publication number 5991-1058EN

Solid Phase Microextraction Accessories

The following accessories are helpful with SPME sample preparation. Select the appropriate accessories for your application needs.

Solid Phase Microextraction Accessories
Description | Part No.
Merlin Microseal | 392609902
SPME replacement seal, 23 gauge, 1/pk |
SPME 15 mL Stand | SU57357U
SPME Fiber Holder for CTC Autosampler | SU57347U
SPME Fiber Holder for Manual Sampling | 391896401
SPME Inlet Guide for Manual Injection - fits most Agilent injection ports | SU57356U
SPME Link Septa, 11 mm | 392548402

Micro-volume SPE

OMIX Tips

• Fast, uniform flow maximizes productivity and reproducibility

• Minimal peptide losses lead to higher recoveries

• Available in three phases and sizes to deliver better sequence coverage

OMIX tips with monolithic sorbent tip technology offer dependable purification and superior results in proteomics research. Agilent OMIX pipette tips reliably purify and enrich femtomole and picomole levels of peptides and proteins prior to MALDI-TOF or LC/MS/MS. The unique monolithic sorbent technology used in OMIX consistently outperforms other tips by delivering uniform flow and strong analyte-to-surface interactions. The high binding capacity of OMIX delivers high productivity – the 10 μL tips bind up to 8 μg of peptide – twice as much as tips from other suppliers. OMIX’s superior flow and exceptional binding capacity ensure reliable recovery of your peptides, minimizing peptide loss during multi-aliquot, multi-tip and evaporation steps.

Omix tips tray, A57009MB

OMIX Tips and Plates for Robotic Automation

• Fast, uniform flow maximizes productivity and reproducibility

• Small monolithic tip delivers low elution volumes, increasing sensitivity and reducing solvent usage

• Vacuum-free processing improves reproducibility and shortens processing times

OMIX 96-well VersaPlate

OMIX automation-friendly 96-well monolithic SPE plates are specially designed to process small samples. They offer small extraction beds with almost no dead volume. Elution is achieved with microliter solvent volumes, allowing direct injection and improving assay speed and sample throughput. OMIX tips are highly amenable to ADME/DMPK bioanalysis applications.

OMIX 96-well VersaPlate Formats
Description | Part No.
OMIX 96-well VersaPlate, C4 with tubes | A57109
OMIX C4 tubes only, 96/pk* | A57109A
OMIX 96-well VersaPlate, C18 with tubes | A57103
OMIX C18 tubes only, 96/pk* | A57103A
OMIX 96-well VersaPlate, MP1 with tubes | A57111
OMIX MP1 tubes only, 96/pk* | A57111A

*Tubes need to be inserted into a VersaPlate base plate, P/N 75400000

OMIX Tips for Tomtec Quadra

Tomtec-compatible tips contain a slice of monolithic SPE material, allowing for vacuum-free processing and walk-away automation. With hands-free SPE, the process becomes much more streamlined and reproducible.

OMIX Tips for Tomtec Quadra
Description | Unit | Part No.
OMIX C18 | 5 racks x 96 tips | A57303
OMIX MP1 | 5 racks x 96 tips | A57311

OMIX C18 for Tomtec Quadra, A57303

Close-up of OMIX tips for Tomtec Quadra

OMIX Tips for Hamilton Microlab STAR Line

Offering excellent versatility and end-user productivity enhancements, these tips have an operating volume of 300 μL, allowing flexibility in sample size. Processing 96 samples can be reduced to just a few minutes in certain applications.

OMIX C18 for Hamilton 300 μL, A57403

Close-up of OMIX tips for Hamilton

OMIX Tips for Hamilton Microlab STAR Line, 300 μL
Description | Unit | Part No.
OMIX C18 | 5 x 96 tips | A57403
OMIX MP1 | 5 x 96 tips | A57411

OMIX Tips for Hamilton STAR, MP1, 5 mg

Disk SPE Formats

Bond Elut SPEC SPE

• No loose sorbent means no channeling of sample

• Uniform flow and extraction properties offer robust performance

• Low elution volume affords excellent concentration of analyte, improving sensitivity

Using an advanced disk design, Bond Elut SPEC delivers superior flow characteristics and trouble-free automation. Due to the low volume of the extraction bed, very low elution volumes can be used. This means that, in some applications, evaporation and reconstitution steps can be eliminated, resulting in accelerated sample processing times. The combination of low bed masses, ultra-clean base materials and a broad toolbox of selectivities delivers higher recoveries free of the matrix interferences that can cause ion suppression.

SPEC provides high recoveries at low elution volumes – as low as 100 μL. This is due to the very high surface area yet small physical volume of the monolithic disk. Overall, extraction efficiency is very high for this format of sample preparation product, and the range of functionalities allows fast method development. SPEC extraction methods are typically shorter and require less reagent and solvent than other SPE methods, for lower costs and greener operation.

SPEC 47 mm disks and SPEC SPE cartridges, A74702

Unique phases available in SPEC 96-well and SPE tube formats

Uniform recovery and reproducibility between wells from the same well plate

• DAU – This functionalized SPEC disk is specifically designed for the forensic analysis of drugs in urine. Its unique sorbent chemistry results in excellent sample cleanup and concentration of samples prior to GC/MS and LC/MS.

• MP1 – SPEC MP1 is a mixed-mode, non-polar/SCX monolithic disk ideal for analytes with polar functional groups in plasma. The dual retention mechanism results in cleaner extracts. The SCX functionality strongly binds polar basic analytes allowing rigorous washing steps to be employed. Bond Elut Certify offers similar selectivity to SPEC MP1.

• MP3 – SPEC MP3 is slightly more polar than MP1, making it ideal for hydrophobic analytes that would bind too strongly to MP1. MP3 chemistry is particularly suited to the extraction of opiate alkaloids from biological fluids.

Note the high recovery (y axis) with an average deviation across the 96 wells of just 3.2% (well positions are shown on the x and z axes). SPEC provides the predictable flow characteristics analysts require for true walk-away automated processing. With SPEC you need not worry about clogging, and as an added benefit, the typically low vacuum pressure requirement prevents cross-talk (e.g. spraying of fast running eluates between wells in the collection plate).

SPEC 96-well Plates

When used on an automated platform, SPEC 96-well plates offer outstanding flow characteristics. Flow across all 96-well plates is uniform and highly reproducible, meaning your recoveries are too.

SPEC 96-well plates

SPEC 96-well Plates, 15 mg
Sorbent Phase | Part No.
Silica-based Sorbents |
C18 | A59603
C18AR | A59619
C18AR, 30 mg | A5960330
C2 | A59601
C8 | A59602
CN | A59606
DAU | A596DAU
NH2 | A59607
Phenyl | A59610
Ion Exchange Sorbents |
SAX | A59605
SCX | A59604
Mixed Mode Sorbents |
MP1 | A59611
MP3 | A59620
Method Development Plate |
C2, C8, C18, C18AR, CN, MP1, MP3, PH | A59630

SPEC SPE Cartridges

SPEC functionalities are also available in a standard straight barrel tube format, offering flexibility in sample size. Use on any standard vacuum manifold such as the Vac Elut 20 or SPS 24.

SPEC SPE C18 cartridges, A5320320

SPEC SPE Cartridges, 100/pk
Sorbent Phase | Description | Part No.
C18 | 15 mg, 3 mL | A5320320
| 30 mg, 3 mL | A5320330
C18AR | 15 mg, 3 mL | A5321920
| 30 mg, 3 mL | A5321930
| 35 mg, 10 mL | A5021935
C18AR/MP3 | 70 mg, 10 mL | A5022570
C2 | 30 mg, 3 mL | A5320130
C8 | 15 mg, 3 mL | A5320220
| 30 mg, 3 mL | A5320230
DAS | 15 mg, 3 mL | A532DAS
DAU | 15 mg, 3 mL | A532DAU
MP1 | 15 mg, 3 mL | A5321120
| 30 mg, 3 mL | A5321130
| 35 mg, 10 mL | A5021135
| 70 mg, 10 mL | A5021170
MP3 | 15 mg, 3 mL | A5322020
| 30 mg, 3 mL | A5322030
| 35 mg, 10 mL | A5020735
NH2 | 15 mg, 3 mL | A5320720
| 70 mg, 10 mL | A5020770
Phenyl | 15 mg, 3 mL | A5321020
| 30 mg, 3 mL | A5321030
SAX | 15 mg, 3 mL | A5320520
| 30 mg, 3 mL | A5320530
| 35 mg, 10 mL | A5020535

SPEC 47 mm disks and SPEC SPE cartridges, A74702

SPEC Disks and Accessories
Description | Part No.
SPEC disks, C18AR, 47 mm, 20/pk | A74819
SPEC disks, C18AR, 90 mm, 12/pk | A79019
SPEC disks, C8, 47 mm, 24/pk | A74702
SPEC environmental disk holder, 47 mm | A713
SPEC flask, 1 L, male 40/35 ground glass fitting | A714

Empore Disk SPE

• Good flow of large sample volumes

• Range of versatile sorbent chemistries

• Available in two disk diameters for better performance

Empore extraction disks provide a high flow rate solution for large volume sample preparation, and are available in a variety of bonded phases and two diameters, 47 and 90 mm. Increasing the diameter of the disk gives better solvent flow rates through the disk.

Empore Disk SPE
Description | Unit | Part No.
Anion extraction disks, 47 mm | 20/pk | 12145012
Chelating extraction disks, 47 mm | 20/pk | 12145029
SDB-XC extraction disks, 47 mm | 20/pk | 12145010
C8 extraction disks, 47 mm | 20/pk | 12145002
C18 extraction disks, 47 mm | 20/pk | 12145004
C18 extraction disks, 90 mm | 10/pk | 12145007

Anion extraction disks, 47 mm, 12145012

TIPS & TOOLS Maximum Binding Capacity of SPEC discs or Empore Disks is 10% of the sorbent bed mass. | [picture]

Bulk SPE

Bondesil Bulk Sorbents

• Ideal for dispersive cleanup techniques

• Advanced bonding offers reproducible batch-to-batch performance

• Multi-kilo quantities available upon request

Bondesil Alumina-N bulk sorbent, 12213073

QuEChERS

Agilent Bond Elut QuEChERS Kits make sample prep as easy as 1- 2- 3. Pre-packaged Agilent Bond Elut QuEChERS Kits are an easy way to capture the time-saving benefits of QuEChERS sample preparation.

• Extraction kits with pre-weighed anhydrous salts in sealed packets allow you to add salts after you add organic solvent to your sample – minimizing an exothermic reaction that can compromise analyte recovery

• Dispersive kits with sorbents and salts supplied in 2 mL or 15 mL centrifuge tubes accommodate the aliquot volumes specified by current AOAC and EN methodologies

• Universal dispersive kits provide excellent recoveries and reproducibility for all types of fruits and vegetables

• Ceramic homogenizers break up salt agglomerates, promoting consistent sample extraction and increasing product recovery during extraction and dispersion; shaking time reduced from 60 to 20 seconds

[picture] | TIPS & TOOLS For more information on QuEChERS, please view our webinar “QuEChERS 101: The Basics and Beyond” at www.agilent.com/chem/quecherswebinar

Agilent Recommended Standard Operating Procedure for QuEChERS

In just 3 easy steps, you can prepare any fruit or vegetable sample for multi-class, multi-residue pesticide analysis.

Selection criteria

• QuEChERS method

• Compounds for screening

Use buffered kits if base-sensitive pesticides are present. Agilent recommends using buffered kits as a first choice.

Selection criteria

• QuEChERS method

• Food type to be analyzed

• Aliquot volume

QuEChERS Extraction Kits

• Available with or without 50 mL centrifuge tubes and caps

• Include MgSO₄, NaCl, or other salts for buffering; pre-weighed in anhydrous packet

QuEChERS AOAC 2007.01 extraction kit, 5982-5755

Step 1: Extraction

Choose the extraction salt packet based on your method of analysis, AOAC or EN. The buffered extraction salts are amenable for more labile pesticides. Adding solvent and then salts to a comminuted fruit or vegetable sample (10 g or 15 g) enables you to extract the pesticides of interest into the organic layer. Agilent pre-packages its QuEChERS salts and buffers in anhydrous packages. This allows you to add them after adding your solvent to the sample, as specified in QuEChERS methodologies.

In the table below, the “CH” products contain the appropriately sized CH for those particular kits. For more information on Ceramic Homogenizers See Here.

Ceramic homogenizer for 50 mL tubes, 5982-9313

QuEChERS Dispersive Kits

Step 2: Dispersive SPE Cleanup

Select the Dispersive SPE kit suited to the type of food being analyzed and the method you are following. In this step, an aliquot of the sample extract from Step One is added to a 2 mL or 15 mL centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning. Select kits are now available with ceramic homogenizers (2 per tube). Their part numbers are designated by a CH.

QuEChERS dispersive kit, 5982-5022

QuEChERS dispersive kit, 5982-5022CH

TIPS & TOOLS View the core concepts surrounding the QuEChERS method at www.agilent.com/chem/QuEChERSvideo | [picture]

[picture] | TIPS & TOOLS Acccess the complete QuEChERS applications library at www.agilent.com/chem/QuEChERS

QuEChERS Ceramic Homogenizers

Ceramic homogenizers increase your overall lab productivity and give you greater confidence in your results. They make analyte extraction easier by:

• Cutting the required extraction time from 60 seconds to as little as 20 seconds – a time savings of 70% per sample

• Maintaining high, reproducible extractions in a third of the time

• Minimizing variance between technicians

• Breaking up salt agglomerates and maintaining a consistent grinding of homogenizing material

The same great ceramic homogenizers available in our QuEChERS Kits are also available for bulk purchase, providing excellent grinding capabilities of the samples.

QuEChERS Ceramic Homogenizers
Description | Unit | Part No.
Ceramic homogenizer for 50 mL tubes | 100/pk | 5982-9313
Ceramic homogenizer for 15 mL tubes | 100/pk | 5982-9312
Ceramic homogenizer for 2 mL tubes | 200/pk | 5982-9311

Ceramic homogenizer for 50 mL tubes, 5982-9313

Standards for QuEChERS Products

In addition to our industry-leading QuEChERS Kits, Agilent makes your analysis easier by providing standards for the most commonly used regulatory methods, including AOAC and EN.

• Save time and avoid inconvenience of making standards

• Available for both GC and LC instruments

• Ready to use for QuEChERS extractions – no dilutions required

Captiva Filtration

Captiva’s unique dual-depth filtration media provides complete removal of precipitated proteins and outstanding resistance to sample clogging, with no loss of analytes. All Captiva components are ultra clean, and rigorously tested to ensure against non-specific binding. With Captiva, your plasma samples are processed quickly and reliably. Captiva is easily automated for enhanced productivity and excellent for sample storage.

Time-consuming sample transfer steps required with conventional precipitation are now a thing of the past. With Captiva, clean, clear filtrates are ready for injection in minutes – this user-friendly filtration device is simple and streamlined with an easy-to-follow 3-step process. And because Captiva samples are pellet-free, you can sample directly from the collection plate.

The Captiva range includes:

• Captiva ND non-drip filtration plates for organic-based protein precipitation

• Captiva NDLipids non-drip filtration plates for lipid and protein depletion

• Captiva 96-well filter plates for general sample filtration

• Captiva filter cartridges, all the usual Captiva benefits in a standard SPE cartridge format

• Captiva Syringe Filters available in a wide range of sizes, formats, and membranes to cover every matrix and sample

Capitiva ND 96-well plate, A5969045

Captiva ND

A simple-to-use filtration device designed for high-throughput, automated, in-well protein precipitation. Built with a unique non-drip (ND) membrane, Captiva ND plates allow for solvent-first protein precipitation using methanol or acetonitrile. Captiva’s unique dual filter design offers fast uniform flow while avoiding sample loss and filter plugging.

Captiva NDLipids

Specifically designed for LC/MS bioanalysis of plasma, Captiva NDLipids combine the ease of use and superior flow properties of Captiva ND with a unique chemical filter. The plate efficiently removes ion-suppressing phospholipids, proteins, and surfactant interferences from precipitated plasma samples.

Captiva Syringe Filters

Captiva Syringe Filters reliably filter from 1mL up to 150mL sample volume for HPLC, UHPLC, CE, ICP-MS and LC/MS with superior flow rates and maximum loading capacity to ensure maximum productivity.

All products are supplied with an HPLC or LC/MS Certificate guaranteeing extremely low levels of extractables. Packages are color coded by membrane for easy and fast identification.

Premium syringe filter, glass microfiber, 5190-5122

Captiva ND

• Easy automation – non-drip design resists organic solvent flow until vacuum is applied

• Exceptional flow – dual depth filter avoids plugged membranes and lost samples

• Efficient protein removal – MS-suitable samples in as little as one-fifth the time

• Multiple pore sizes available for greater flexibility with solvent use

Captiva ND’s unique non-drip design simplifies your workflow, ends the need to use messy tip or well seals, and reduces the number of liquid transfer steps needed to process samples. Best of all, Captiva ND’s dual-depth filter construction delivers a fast reproducible flow, so you get uniform sample treatment and reliable filtrate recovery in a fraction of the time of other protein precipitation plates.

Captiva ND 96-well filter plates
Description | Unit | Part No.
Capitiva ND plate, 0.2 μm, polypropylene Recommended for both methanol and acetonitrile | 5/pk | A5969002
Capitiva ND plate, 0.45 μm, polypropylene Suitable for acetonitrile only | 5/pk | A5969045

Get fast, reproducible flow with Agilent Captiva ND

Agilent Captiva ND plates process samples quickly and uniformly without the need for positive pressure manifolds. Plus, its uniform flow facilitates vacuum processing making it more automation friendly.

TIPS & TOOLS For more information on Agilent Captiva ND Plates, please visit www.agilent.com/chem/captiva | [picture]

Captiva NDLipids

• More precise and reproducible quantitation with removal of phospholipids and proteins

• Increased productivity due to extended column lifetimes and cleaner MS ion sources

• Simple 3-step procedure

• Available with 0.2 μm pore size only, to optimize lipid removal; Methanol recommended

Capitiva NDLipids is as simple and easy-to-use as a standard protein precipitation plate. The non-drip 96-well filtration plate is specially designed to effectively remove phospholipids from biofluids. Captiva NDLipids removes lipids, proteins, surfactants and other matrix interferences from plasma extracts. Ion suppression is significantly reduced for enhanced sensitivity and precision during trace analysis. The depletion of lipid compounds also gives you better peak shapes and reproducible retention times so that standard operating procedures are easily validated. In addition, the fast, in-well precipitation technology of Captiva NDLipids ensures high sample throughput and helps reduce instrument downtime, with virtually no need for method development on a wide range of analytes.

Captiva NDLipids 96-well filtration starter kit, A59640002SK

Captiva NDLipids 96-well filter plates
Description | Part No.
Captiva NDLipids 96-well filtration starter kit Includes 1 CaptiVac vacuum collar, 2 Captiva NDLipids filter plates, 2 Captiva 96 deep-well 1 mL collection plates and 2 Captiva collection plate pierceable covers | A59640002SK
Captiva NDLipids 96-well filtration replacement kit Includes 2 Captiva NDLipids filter plates, 2 Captiva 96 deep-well 1 mL collection plates and 2 Captiva collection plate pierceable covers | A59640002RK
Captiva NDLipids 96-well filtration plate, 100/pk | A59640002B
Captiva NDLipids 96-well filter plate, 1 mL well, 1/pk | A59640002I
Captiva NDLipids 96-well filter plates, 1 mL well, 5/pk | A59640002V
DuoSeal 96 96-well plate seals, 10/pk | A8961008

TIPS & TOOLS

Using Captiva NDLipids with methanol is an excellent replacement for acetonitrile as the precipitation solvent. Methods with methanol show better removal of lipids than with acetonitrile. Converting to methanol is advantageous when the supply or cost of acetonitrile is restrictive. Methanol can now be your solvent of choice for lipid removal.

For more information about solvents, reference Application Note “Agilent Captiva ND Lipids Sample Prep Choice of Precipitation Solvent: Acetonitrile versus Methanol” publication number 5991-0445EN.

TIPS & TOOLS

Agilent provides you with the tools you need to make bioanalysis quick and reliable. Here, in this video, we demonstrate an opiate panel analysis, from sample prep using Captiva NDLipids through HPLC separation using Poroshell 120 columns and the Agilent 6490 QQQ MS/MS with iFunnel. For part one of this video, please visit www.agilent.com/chem/bioanalysis1 – for part two of this video, please visit www.agilent.com/chem/bioanalysis2

Operating Instructions and Tips for Captiva NDLipids 96-well Plates

Post-Column Infusion (PCI) of Albuterol Before Treatment With Captiva NDLipids

Note that the ion-suppression features (top trace) correlate with the elution of phospholipids (bottom trace).

Same Experiment After Protein and Lipid Depletion With Captiva NDLipids

Ion suppression is dramatically reduced and the lipids are almost non-detectable.

Longevity Study Illustrating Prolonged Column Lifetime When Using Captiva NDLipids

No significant changes in back pressure, retention time, and peak shape with Captiva NDLipids after 10 and 5010 injections for LC/MS or LC/MS/MS bioanalysis (top = UV detection; bottom = MS detection).

Captiva 96-well Filter Kits

• The industry standard for centrifugation-free sample filtration

• Fast and reliable processing improves productivity

• Starter kits contain everything you need

Faster than centrifugation and easily automated, Captiva’s unique dual-depth filtration media provides outstanding resistance to sample clogging. With Captiva, your samples are processed quickly and reliably, and you can avoid fibrinogen clogging forever. The plates are also excellent for sample storage. All Captiva components are ultra clean, and rigorously tested to ensure against non-specific binding. Starter kits contain everything you need to get up and running with minimum fuss. Replacement kits include everything you need to replenish your Captiva system.

Captiva 96-well Filter Kits

Captiva 96-well Filter Kits
Pore Size (μm) | Filter Material | Part No.
Starter Kits |  |
0.2 | Polypropylene | A5960002SK
0.45 | Polypropylene | A5960045SK
Includes 1 CaptiVac vacuum collar, 5 Captiva filter plates, 10 DuoSeal 96 96-well plate seals, 5 Captiva 96 deep-well 1 mL collection plates, 5 Captiva collection plate pierceable covers
Replacement Kits |  |
0.2 | Polypropylene | A5960002K
0.45 | Polyvinyldifluoride and polypropylene | A5967045K
| Polypropylene | A5960045K
Includes 5 Captiva filter plates, 10 DuoSeal 96 96-well plate seals, 5 Captiva 96 deep-well 1 mL collection plates, 5 Captiva collection plate pierceable covers

Captiva 96-well Filter Plates

• Protect HPLC columns from clogging to reduce instrument downtime

• Clean and clear filtrates offer improved sensitivity

• High analyte recovery with simple robust methods allows faster method development

Filtration is simple, versatile, and necessary to prevent clogging of valuable HPLC columns. Captiva 0.2 μm and 0.45 μm depth filter plates are ideal for filtering samples prior to LC/MS injection. Captiva 10 μm and 20 μm glass fiber filter plates are designed for clarifying highly particle-laden samples, such as freshly thawed plasma and hepatocyte filtration, preventing sample transfer problems from pipette tip clogging. They are perfect for automated systems and for use with DuoSeal 96 96-well seals.

Captiva 96-well filter plates, A5960045

Captiva 96-well Collection Plates and Cover

• Designed for Captiva filtration, SPEC and Bond Elut 96 applications

• Standard 1 mL format offers compatibility with further automation or liquid handling

• Silicone cover preserves sample integrity

Captiva 96-well collection plates are specially designed for use with Captiva filtration plates, SPEC SPE 96-well plates and Bond Elut 96-well plates. The 1 mL capacity provides the volume needed to collect all of your filtrate or eluate. Captiva pierceable 96-well silicone covers are easily applied to completely seal the plates, ensuring no sample loss due to spillage or evaporation and no sample contamination. The silicone is specially designed for 96-well auto injectors, providing easy piercing and removal.

Captiva 96-well collection plate, A696001000

Captiva 96-well Collection Plates and Cover
Description | Unit | Part No.
Captiva 96-deep well collection plate, 1 mL | 10/pk | A696001000
Captiva 96-deep well collection plate, 1 mL | 100/pk | A696001000B
Captiva pierceable 96 deep-well collection plate cover, 1 mL | 10/pk | A8961007
DuoSeal 96-well plate seal | 10/pk | A8961008

Captiva Filter Cartridges

• Standard SPE format

• Ideal for LC/MS samples

• Avoid sample transfer problems

• Non-Drip (ND) 3 mL cartridges resist flow until vacuum is applied

• Effectively remove phospholipids from biological samples with Captiva NDLipids

Captiva filter cartridges bring all of the benefits of Captiva filtration to the standard SPE cartridge format. The 0.2 μm and 0.45 μm filter cartridges are ideal for preparing precipitated protein samples for LC/MS analysis. The Captiva 10 μm glass fiber filter cartridge is designed for clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample transfer problems due to pipette tip clogging.

Captiva filter cartridges, glass fiber, A500401000

TIPS & TOOLS

Using Captiva NDLipids with methanol is an excellent replacement for acetonitrile as the precipitation solvent. Methods with methanol show better removal of lipids than with acetonitrile. Converting to methanol is advantageous when the supply or cost of acetonitrile is restrictive. Methanol can now be your solvent of choice for lipid removal.

For more information about solvents, reference Application Note “Agilent Captiva NDLipids Sample Prep Choice of Precipitation Solvent: Acetonitrile versus Methanol” publication number 5991-0445EN.

CaptiVac Vacuum Collar

• Pre-aligned for trouble-free operation

• Vacuum sealed for maximum efficiency

• Simple, cost effective solution

For use with Captiva Filtration and SPEC 96-well Plates, this patented vacuum collar is a completely transparent device that joins Captiva or SPEC plates directly onto our collection plate. The unique design of the Captiva collar forms a pre-set, pre-aligned vacuum seal between the filtration and collection plate, which positions the outlet tips at a specified distance inside each well, so as to prevent cross contamination of samples.

CaptiVac vacuum collar, A796

CaptiVac Vacuum Collar
Description | Part No.
CaptiVac vacuum collar | A796
CaptiVac gasket kit, 5/pk | A796G

Premium Syringe Filters

• More choices. Captiva syringe filters are available in a wide range of sizes, formats, and membranes to cover every matrix and sample.

• Certified. All products are supplied with an HPLC or LC/MS Certificate, guaranteeing extremely low levels of observed extractables.

• Exceptional Flow Rate. Captiva syringe filters have excellent flow rates and maximum sample loading capacity.

• Highest Quality. Agilent Captiva syringe filters are constructed with the highest-grade virgin polypropylene housing, and are securely welded to prevent bursting and ensure sample integrity.

Sample filtration prior to HPLC, LC/MS, UHPLC, CE and ICP-MS analysis is critical to achieving optimal system performance, and Agilent Captiva Premium Syringe Filters make the process faster than ever with the industry’s highest flow rates and loading capacities. Manufactured with the highest-grade virgin polypropylene, and all are HPLC or LC/MS certified to guarantee low levels of observed extractables. PES (part numbers 5190-5094, 5190-5095,5190-5096 and 5190-5098) and Glass Fiber (5190-5120) premium syringe filters are LC/MS certified to be free of extractables Choose from a variety of membranes to suit your needs.

LC/MS Certificate of Analysis

Layered Filters with Pre-Filter

Captiva disposable syringe, 5 mL, 9301-6476

Captiva Disposable Syringes, 100/pk
Volume (mL) | Part No.
5 | 9301-6476
10 | 9301-6474
20 | 5062-8534

Captiva disposable syringe, 10 mL, 9301-6474

Captiva disposable syringe, 20 mL, 5062-8534

Econofilters

High quality Econofilters are shipped in large packs and are ideal for busy labs that need fast, efficient filtration at a reasonable price.

Econofilters, PES, 5190-5272

Agilent Captiva Syringe Filter Selection Guide

Applications

Type of Filtration | Recommended | Alternatives
HPLC • UHPLC • LC/MS • GC | RC | PTFE or Nylon
ICP-MS | PTFE | Glass Fiber/PTFE (High Particle Samples)
CE | RC | Nylon
Undiluted Organic Solvents | PTFE | Nylon
Protein Analysis • Samples with Biomolecules – Buffers | PES | RC or CA
Tissue Culture Media | PES | RC or CA
High Particle-Load Samples – Organic Solvents | Glass Fiber/PTFE |
High Particle-Load Samples – Aqueous Solutions | Glass Fiber/Nylon |

Proof of Performance: Filtration Efficiency

Testing Method

Sample preparation

The surfactant solution, 0.1% Triton X-100, was used to prepare 0.01% Latex Beads (0.3 μm and 0.5 μm) solution. The 0.1% Triton X-100 was used to maintain the homogeneity of Latex Beads solutions.

Filtration

The challenging solution was passed through each individual syringe filter and a 1 mL filtrate was collected in a 2 mL vial for HPLC run. Ten different filters from each kind filter were tested.

Filtrate measuring on HPLC/UV

The maximum absorbance of the latex beads solutions was observed at 272 nm, which was used to correlate latex beads concentration with absorbance.

A simple HPLC method was used for automatic testing under UV 272 nm.

No column was used. The mobile phase was water, and the flow rate of 1.0 mL/min was used.

The eluted peak are at 272 nm was used for filtration efficiency calculation.

Blank 0.1% Triton X-100 was run to correct contributions from surfactant absorbance at 272 nm.

Agilent Captiva Syringe Filters provide equivalent or better filtration efficiency than competitors equivalent products on particulates removal

Proof of Performance: Flow Rate & Volume Capacity

Agilent Captiva Premium Syringe Filters provide incomparable loading capacity with the fastest flow rates in the market today to allow for maximum efficiency.

Filtration Impact on LC Column Life

Importance of Filtration

Column plugging is the most frequent cause of column failure encountered by analytical chemists. Injection of samples containing even small amounts of particulate will clog the column inlet, cause high column backpressure, retention time shift and loss of resolution, and subsequently shorten the normal column lifetime. This impact can be more significant for sub-2 μm columns. These smaller particle size columns are usually used under high pressure, thus are more sensitive to pressure increase caused by the accumulated particulates on column.

It is the intent of this work to demonstrate that sample filtration will lengthen the life of a column, not only the traditional LC columns by 0.45 μm filters, but also the sub-2 micron LC columns by 0.2 μm filters. In order to correlate the column life extend to the actual application, the plasma extracts by PPT treatment were tested also for the comparison of samples without filtration, samples with centrifugation and samples with filtration.

Testing Method

Sample preparation

A.) The surfactant solution, 0.002% Triton X-100, was used to prepare 0.05% Latex Beads (0.3 μm and 0.5 μm) solution.

B.) Latex Beads solution (0.3 μm) was used for sub-2 micron column life test. Unfiltered and filtered (by 0.2 μm filters) samples were used for comparison of impact on sub-2 micron column life.

C.) Human plasma extract was used for sub-2 micron column life actual application test. Unfiltered, centrifuged and filtered (by 0.2 μm filters) samples were used for comparison of impact on sub-2 micron column life. The sample was prepared following the below steps.

1. 2 mL of Human plasma was aliquoted in to a test tube.

2. 10 mL of Acetonitrile with 1% Acetic Acid was added.

3. Sample was vortexed vigorously and then centrifuged at 4000 rpm for 5 min.

4. The supernant was transferred into a clean test tube.

5. The supernant was blown dry with N2 flow at 37 °C.

6. The died sample was reconstituted in 10:90 MeOH/H2O. Vortex and sonicate.

Filtration

The challenging solution was passed through each individual syringe filter and a 1 mL filtrate was collected in a 2 mL vial for HPLC run.

UHPLC instrumentation (for sub-2 column life test)

Column: | Agilent Zorbax Eclipse Plus
| C18 RRHD column,
| 2.1 x 50 mm, 1.8 μm, P/N 959757-902
| Column was disconnected from the detector and allowed to run to drain.
Mobile phase: | Acetonitrile: Water (35:65, v/v)
Flow rate: | 0.4 mL/min, isocratic
Injections: | 10 μL per injection, 1 injection per minute
Monitoring: | Column backpressure was recorded with the number of injections.
Column failure: | When column back pressure exceed 1000 bar.
Sequence: | A 1000 injections sequence was usually used, unless column failed in the middle due to high pressure. A new column was used for each individual sequence

Conclusion

Sample filtration prior to their introduction into an HPLC system was demonstrated to make significant improvement on the column usage life time.

Diatomaceous Earth Sorbents

Chem Elut and Hydromatrix

• High purity sorbent supported liquid extraction (SLE) applications

• Available in pre-packed cartridges or bulk

• Packing method delivers excellent tube-to-tube reproducibility

• Tox Elut cartridges are effective for forensic analysis of drugs in urine

Chem Elut is an economical broad performance sorbent for rapid, general sample preparation of biological samples such as plasma, serum, whole blood and urine. Chem Elut products are available in buffered and unbuffered formats. The buffered devices can be used for simple scrubbing operations on organic reactions. The base-treated cartridge can remove residual acid compounds from a variety of matrices.

Hydromatrix is a high purity, inert diatomaceous earth sorbent available in 96-well plates (Combilute and Chem Elut SLE Plates, which are designed for sample volumes of less than 80 μL) and as bulk material, offering end user flexibility and an excellent diversity of applications.

Typical Matrices

Aqueous, biological fluids, organic reaction mixtures (scavenging)

Primary Extraction Mechanism

Solid supported LLE

Compound Types

Nitrosamines, pesticides, herbicides

Chem Elut cartridges, 12198006

Hydromatrix
Description | Part No.
Hydromatrix bulk material, 1 kg | 198003
Hydromatrix bulk material, 4 kg | 198004

Combilute plate, 200 mg, 65401507

Other Formats*
Description | Part No.
Combilute 96-well plate, 200 mg | 65401507
Chem Elut SLE Plate, 50 mg | A4964050
Chem Elut SLE Plate, 150 mg | A4964150
Preassembled 96-well plate (VersaPlate tubes and base plate) 260 mg | 75430260
VersaPlate tubes, 96/pk, tubes only, 260 mg | 75530260

*Tubes need to be inserted into a VersaPlate base plate, P/N 75400000

References

Plum, J & Daldrup, T (1986) Detection of digoxin, digitoxin, their cardioactive metabolites and derivatives by high performance liquid chromatography and high performance liquid chromatography-radioimmunoassay. J. Chromatogr. A, 377, 221-231.

Biondi, PA, Guidotti, L, Montana, M, Manca, F, Brambilla, G & Lucarelli, C (1991) A derivatization procedure suitable for HPLC analysis of clenbuterol. J. Chromatogr. Sci., 29(5), 190-193.

Raou, S, Gremaud, E, Biaudet, J & Turesky, R (1997) Rapid solid-phase extraction method for the detection of volatile nitrosamines in food. J. Agricultural and Food Chem., 45, 4706-4713.

The prohibition on the use of certain azo dyes is laid down in Annex XVII to the EU Regulation (EC) 1907/2006 on the registration, evaluation and authorization of chemicals (REACH), which is directly applicable in all EU Member States. CEN Leather - Chemical tests - Determination of certain azo colourants in dyed leathers. Reference: CEN ISO/TS 17234:2003

Dried Matrix Spotting

Bond Elut Dried Matrix Spotting (DMS)

Dried blood spotting techniques for DMPK/ADME applications have gained significant exposure in the past few years. The practical advantages of sample collection, shipping and storage offer significant resource benefits to large pharmaceutical and CRO laboratories. Bond Elut DMS resets the bar in performance for dried blood spotting applications. The innovative non-cellulose spotting material offers key sensitivity and workflow advantages, mitigating key pains with existing cellulose blood spotting cards.

• Non-cellulose 'paper' reduces non-specific binding, improving MS analyte response, increasing signal to noise ratios

• Easy product selection – one single, untreated card for fast method development for a variety of biological matrices

• Spot size, homogeneity and recovery are highly reproducible across a range of hematocrit levels: allowing confidence in assay development for a variety of biological matrices

• Non-hygroscopic material does not absorb moisture, even in aggressively humid environments, reduces potential risk of analyte stability during transportation/storage

• Card requires five times less punching force than a cellulose based DBS card, enabling easier workflow and amenability to automation

DMS card, 50/pk, A400150

Bond Elut Dried Matrix Spotting (DMS)
Description | Unit | Part No.
DMS card | 50/pk | A400150
DMS card | 500/pk | A400150K
DMS accessory pack |  | A42001
Includes 5 x 3 mm punching tools and 5 punching mats |  |
DMS starter kit |  | A400150SK
Includes 1 accessory pack (P/N A42001) with 5 x 3 mm punching tools and 5 punching mats and Bond Elut DMS card, 50/pk (P/N A400150) |  |

| TIPS & TOOLS
[picture] | Visit www.agilent.com/chem/sampleprep for Dried Matix Spotting Application Notes, including: "Homogeneity of Dried Matrix Spots," publication # 5990-8035EN.

Dried Blood Spotting Workflow and Method Guide

1. SPOTTING

• Use existing sample collection workflow and preferred blood volume (typically 15 μL, but better MS responses can be achieved using 30 μL)

• Hold the pipette tip or capillary just above the paper in the center of the dotted circle (DO NOT ALLOW THE TIP TO TOUCH THE CARD SURFACE)

• Dispense, allowing the blood drop to touch the spotting surface on the card

• The blood spot will quickly soak into the surface of the card leaving a circular spot

2. DRYING

• Drying times for Bond Elut DMS cards are comparable to cellulose based cards

• It is recommended that the sample is given a minimum of 2 hours drying time prior to punching

3. PUNCHING

The Agilent DMS card is compatible with handheld punch tools and can easily be configured for automated punching systems compatible with a 4 spot card format.

• Cut a suitable sized punch from the center of the blood spot (typically 3 mm)

• Transfer the punched blood disk to a suitable vial or 96-well

4. EXTRACTION

• Add 300 μL of 0.1% formic acid* in 80% methanol to the punched disk

• Add a suitable internal standard to the sample and vortex

• Centrifuge for 15 minutes or filter if required

• Evaporate sample to dryness and reconstitute in 100 μL of mobile phase

• Inject into LC/MS/MS

*The use of 0.1% formic acid can assist the elution of more hydrophobic analytes

OTHER PRACTICAL CONSIDERATIONS

Blood Sample: Fresh, untreated blood can be used provided it shows no sign of clotting. In most cases it is common to use blood that contains an anticoagulant (EDTA or heparin). Frozen blood samples are not recommended due to the occurrence of cellular damage upon thawing.

Oval Spots: Oval spots can be caused by sudden hand movement during the spotting process. This does not create any experimental issues, as the subsequent punch from the center of the spot will normalize the assay volume.

Hematocrit Levels: Bond Elut DMS displays improved spot homogeneity and recovery compared to cellulose devices, across a wide range of hematocrit levels.

Punching Force: Bond Elut DMS cards require 4 times less punching force than a cellulose based card. There is no need to twist the cutting tool while punching the blood spot.

Cross Contamination: Provided that the blood spots have had sufficient time to dry, then cross contamination should not be an issue.

Effect of Humidity: Bond Elut DMS cards are non-hygroscopic and do not absorb water, even in high humidity environments.

Untreated Card: Bond Elut DMS contains no chemical impregnation.

Other Matrices: Bond Elut DMS is amenable to a broad range of biological matrices including plasma.

TIPS & TOOLS |
To view Agilent application note "Improving Sensitivity of Basic Drugs in Dried Blood Spotting through Optimal Desorption" please visit www.agilent.com/chem/driedbloodspotting | [picture]

Chromatography Papers

Chromatography Paper is used in thin layer chromatography applications such as evaluating radioisotope purity. The porous paper is made of glass microfibers impregnated with silica gel. Agilent offers two kinds of paper: SA (contains sodium salt) and SG (contains potassium salt).

ITLC SG paper, SGI0001

• More convenient with faster developing times than traditional TLC; no interference from organic binders

• Ideal for evaluating radioisotope QC testing

• Separates lipids and other non-polar compounds

• Can easily be cut to convenient testing sizes, and can be imprinted

Chromatography Papers
Description | Part No.
Chromatography paper (SA), 4.5 x 12 in, 50/pk | A120B12
ITLC SG paper, 4.5 x 12 in, 50/pk | SGI0001

Bond Elut Accessories

Bond Elut 96-well Accessories

Bond Elut 96-well manifold, acrylic, 5133000

Bond Elut 96-well Accessories
Description | Unit | Part No.
96-well manifold, acrylic | 1/pk | 5133000
96-well manifold, shimset | 1/pk | 12236104
Square-well collection plates, 2 mL | 50/pk | 5133009
Square-well collection plates, 1 mL | 50/pk | 5133008
Square-well collection plates, 350 μL | 50/pk | 5133007
Sealing tape pad | 10/pk | 12143105
Square 96-well sealing caps, EVA, pierceable | 50/pk | 5133005
VersaPlate sealing strips, each covers one column | 240/pk | 12236108

96-well manifold, shimset, 12236104

Bond Elut 96 square-well plate, 5133009

Bond Elut 96 square-well plate, 5133008

Bond Elut 96 square-well plate, 5133007

Sealing tape pad, 12143105

Bond Elut Empty SPE Cartridges

• Made with high purity polypropylene for cleaner extracts

• Uniform batch-to-batch size for consistent performance

• Economical for everyday use

Empty SPE cartridges, 1 mL, 12131007

Empty SPE cartridges, 12 mL, 12131010

Empty SPE cartridges, 20 mL, 12131011

Empty SPE cartridges, 60 mL, 12131012

A variety of empty reservoirs is available for packing custom SPE cartridges with bulk Bondesil or other desired sorbents. Cartridges are available from 1 to 60 mL. Order frits separately, or see the table for reservoirs with pre-installed frits.

Bond Elut Empty SPE Cartridges
Volume (mL) | Unit | Part No.
1 | 100/pk | 12131007
3 | 100/pk | 12131008
6 | 100/pk | 12131009
12 | 100/pk | 12131010
20 | 100/pk | 12131011
60 | 100/pk | 12131012

Bond Elut Empty SPE Cartridges with Two Frits

• Pre-installed frits for ease-of-use

• Broad range of filtration operations for maximum flexibility

• Customizable packing for specific applications

Empty SPE cartridges with two frits (pre-inserted), 1 mL, 12131013

These clean polypropylene reservoirs contain two 20 μm polylethylene frits pre-inserted, an ideal configuration for simple filtration. For custom sorbent packing, additional frits can be purchased separately. Available from 1 to 60 mL.

Bond Elut Empty SPE Cartridges with Two Pre-Installed Frits
Volume (mL) | Unit | Part No.
1 | 100/pk | 12131013
3 | 100/pk | 12131014
12 | 100/pk | 12131016
20 | 100/pk | 12131017
60 | 100/pk | 12131018
Bond Elut Empty SPE Cartridges with One Thick Frit |  |
6 | 100/pk | 12131015

Empty SPE cartridges with two frits (pre-inserted), 20 mL, 12131017

Empty SPE cartridges with two frits (pre-inserted), 60 mL, 12131018

20 μm Polyethylene Frits for SPE Cartridges

• Made with high-grade, clean polyethylene for clean extracts

• Pre-cut to correct size for accuracy

• Use with reservoirs or custom packing

These frits are pre-cut to fit into Bond Elut reservoirs for use in filtration applications or for custom SPE sorbent packing.

Polyethylene Frits, 12131021

Bond Elut Adapters

• Connect SPE cartridges in series for large samples

• Expand cartridge volume for even more applications

• Transfer large-volume samples to any SPE cartridge

Bond Elut Adapters
Description | Unit | Part No.
Adapter cap for 1, 3 and 6 mL Bond Elut cartridges | 15/pk | 12131001
Adapter cap for LRC 12, and 20 mL Bond Elut cartridges | 10/pk | 12131003
Adapter cap for 60 mL Bond Elut cartridges | 10/pk | 12131004

Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge, allowing the following configurations:

Bond Elut Adapter Configurations

Configuration 1: | Stack two cartridges to perform multi-sorbent methods
Configuration 2 + 3: | Increase any cartridge's volume by stacking an empty reservoir on top of the device.
Configuration 4: | Standard Luer-tipped syringes will fit into any Bond Elut adapter. Gentle pressure can then be used to apply conditioning solvents, samples, rinsing solvents and eluents. This configuration is particularly useful for single sample processing, where a vacuum manifold is not required.
Configuration 5: | For excessively large sample volumes, 1/8 in od tubing can be connected to the end of an adapter and the sample can be drawn directly from the sample container via high vacuum.

Luer Stopcocks

• Control flow rates during SPE vacuum extraction

• Improve method reproducibility

• Instant isolation from vacuum reduces accidental tube drying

Luer stopcocks are used to provide independent flow control of each individual Bond Elut cartridge when used with vacuum manifolds. They are made from solvent resistant high-grade polypropylene, are reusable and can be readily cleaned using organic solvents such as methanol or acetone.

Luer stopcocks, 12131005

Luer Stopcocks
Description | Unit | Part No.
Luer stopcocks | 15/pk | 12131005

Adapter Caps for Gilson ASPEC SPE Systems

• Enhance the high-throughput compatibility of Bond Elut cartridges

• Converts 1, 3 and 6 mL cartridges for use in Gilson SPE systems

• Specially engineered for leak-free operation

Gilson-engineered caps produce a positive pressure seal with the needle in Gilson ASPEC, ASPEC XL and ASPEC XL4 solid phase extraction systems.

Gilson adapter cap, 12131034

ASPEC Adapter Caps
Description | Unit | Part No.
Gilson adapter cap, 1 mL, yellow | 1000/pk | 12131034
Gildson adapter cap, 3 mL, blue | 1000/pk | 12131035

Vac Elut Vacuum Extraction Manifolds

• Increased productivity/sample throughput

• Disposable needles eliminate cross contamination

• Rugged, reliable construction

Engineered to increase laboratory productivity, the corrosion-resistant Vac Elut vacuum extraction manifolds permit extraction of up to 12 or 20 samples at one time, for improved efficiency. The manifold’s clear glass base allows careful monitoring of the entire sample collection process, and the compact design requires very little bench space.

To minimize the risk of sample carryover, low-cost, disposable, medical grade polypropylene delivery needles can be easily replaced. Polypropylene extender tips are also available as a replacement for the standard needle valves, ensuring a direct path into the collection tube. Correct sample identification is also ensured by an interlocking fit between the lid and internal test tube rack.

Vac Elut 20 Vacuum Extraction Manifolds

The Vac Elut 20 vacuum control valve, vacuum gauge, and quick release valve are mounted on the lid, away from the corrosive waste stream and within convenient reach. The solvent-resistant polypropylene rack is available in a variety of sizes to accommodate collection tubes commonly used in sample preparation. Manifold sets include the glass basin, lid cover, collection rack and vacuum gauge assembly.

Vac Elut 20 manifold with collection rack, 12234105

Vac Elut 20 collection rack, 12234517

Vac Elut 20 Manifold
Manifold Sets | Part No.
Vac Elut 20 manifold with collection rack for 10 x 75 mm test tubes | 12234105
Vac Elut 20 manifold with collection rack for 13 x 75 mm test tubes | 12234100
Vac Elut 20 manifold with collection rack for 13 x 100 mm test tubes | 12234101
Vac Elut 20 manifold with collection rack for 16 x 75 mm test tubes | 12234102
Vac Elut 20 manifold with collection rack for 16 x 100 mm test tubes | 12234103
Accessories for Vac Elut 20 Manifold |
Standard glass basin | 12234505
Collection rack for 10 x 75 mm test tubes | 12234517
Collection rack for 13 x 75 mm test tubes | 12234507
Collection rack for 16 x 100 mm test tubes | 12234510
Replacement Components |
Polypropylene delivery needles, 25/pk | 12234511
Replacement exit valve for glass basin | 12234506
Replacement lid gasket | 12234502
Vac Elut 20 lid cover | 12234501
Vacuum gauge assembly | 12234504

Vac Elut 20 Manifold with Tall Glass Basin

• For extractions greater than 10 mL

• Transparent glass base allows you to monitor the whole collection operation

• Simple vacuum adjustment

The Vac Elut 20 with a large glass basin and collection rack accommodates larger 16 x 150 mm test tubes. The same high quality material and features on the standard Vac Elut system are incorporated on this special unit. These collection vessels can be utilized in combinatorial chemistry applications using large boiling tubes for collection of purified synthesis mixtures, or for any SPE extraction in which an elution volume greater than 10 mL is required.

Vac Elut 20 Manifold with Tall Glass Basin
Manifold Set | Part No.
Vac Elut 20 Manifold with tall glass basin and collection rack for 16 x 150 mm test tubes, complete system | 12234104

Vac Elut 20 manifold tall glass basin, 12234104

Vac Elut 12 Manifold

The Vac Elut 12 vacuum extraction manifold is a compact tool for small sample sets. The Vac Elut 12 offers the same durability of components and operation as the Vac Elut 20 manifolds, but works well when only a few samples need to be processed at a time. The Vac Elut has 12 sample positions, a clear glass basin for easy visualization of the extraction, and a gauge for precise vacuum settings.

Vac Elut 12 manifold, 5982-9110

Vac Elut 12 Manifold
Manifold Set | Part No.
Vac Elut 12 manifold with collection rack for 16 x 100 mm test tubes | 5982-9110

12-port rack for 13 x 75 mm tubes, 5982-9114

Replacement Parts for Vac Elut Vacuum Manifolds
Description | Part No.
Manifold ball ring/vacuum quick release | 5982-9106
Manifold exit valve replacement kit | 5982-9107
Manifold vacuum gauge assembly with valve | 5982-9108
White cover for 12-port manifold | 5982-9111
Sealing gasket for 12-port manifold | 5982-9112
Glass chamber for 12-port manifold | 5982-9113
12-port rack for 13 x 75 mm tubes | 5982-9114
12-port rack for 13 x 100 mm tubes | 5982-9115
12-port rack for 16 x 75 mm tubes | 5982-9116
12-port rack for 16 x 100 mm tubes | 5982-9117

Parts and Disposables for Vac Elut Cartridge Manifolds
Description | Unit | Part No.
Disposable needle tip | 20/pk | 5982-9100
Stainless steel needle with polypropylene coating | 20/pk | 5982-9101
Short valve stopcock | 20/pk | 5982-9102
Long valve stopcock | 20/pk | 5982-9103
Male luer plugs | 25/pk | 5982-9104
Needle tip ejector tool |  | 5982-9105
Cartridge stacking adapters | 12/pk | 5982-9109

Vac Elut SPS 24 Manifold

• Closed operation prevents cross contamination

• Stainless steel tips deliver maximum extract purity

• Range of rack sizes covers most tube configurations

The Vac Elut SPS 24 allows simultaneous processing of up to 24 SPE cartridges. Like all Vac Elut manifolds, the SPS 24 is made from durable, solvent-resistant materials and engineered to last. The glass sides allow easy viewing of the entire sample collection process.

The ultimate feature of the SPS 24 manifold is its waste diversion funnel, which enables all steps of the SPE procedure to be completed without removing the lid. Since the collection rack is placed inside the unit before extraction begins, splash back and cross contamination are eliminated, while hazardous waste and biohazard exposure are minimized. Wastes collect outside of the manifold itself, simplifying cleanup and reducing the time needed to extract and elute samples.

Complete with replacement stainless steel delivery tips for maximum extract purity, the Vac Elut SPS 24 system also includes a vacuum controller/release, collection rack, and port sealing plugs. Racks for several different collection tube configurations are available.

Vac Elut SPS 24 manifold

Vac Elut SPS 24 Manifold
Description | Part No.
Vac Elut SPS 24 manifold with collection rack for 10 x 75 mm test tubes | 12234003
Vac Elut SPS 24 manifold with collection rack for 12 x 75 mm test tubes | 12234041
Vac Elut SPS 24 manifold with collection rack for 13 x 100 mm test tubes | 12234022
Vac Elut SPS 24 manifold with collection rack for 16 x 100 mm test tubes | 12234004
Replacement Components |
Collection rack and funnel set for 12 or 15 mL conical tubes | 12234027
Collection rack and funnel set for 12 x 75 mm test tubes | 12234030
Collection rack and funnel set for 13 x 100 mm test tubes | 12234031
Collection rack and funnel set for 16 x 100 mm test tubes | 12234028
Elastic lid fasteners, 6/pk | 12234034
Complete Upper Lid Assembly | 12234025C
SPS 24 upper lid cover | 12234025
SPS 24 waste tower repair kit | 12234005
Includes base exit tube, hose connector, washer, center tube, 900 connector elbow |
Stainless steel delivery needles, 25/pk | 12234038

SPS 24 waste tower repair kit, 12234005

96-Well Plate Vacuum Manifold Accessories

• Can handle 96-well fixed position plates or second version to handle 96-well flexible format plate

• Constructed with polypropylene base and polyethylene lid

• Small footprint

• Supplied with on/off valve, vacuum gauge, and fine vacuum control valve

• Disposable reservoir tray collects excess sample and wash solvents

• Spacer inserts can be placed into the base so that collection plates of differing heights can be processed (both deep-well and standard microtiter plates), ensuring maximum penetration of the SPE plate into the collection plate and reducing well-to-well contamination

• Resistant gasket in the manifold lid

Base O-ring, 5185-5779

Vacuum Manifolds for 96-well Plates
Description | Part No.
Manifold for 96-well plates | 5185-5776
Includes base, vacuum gauge, needle valve and fixed lid |

96-well vacuum manifold, base assembly only, 5185-5797

Collection plate spacer in sizes to match the collection plate used

Parts and Disposables for 96-well Plate Manifolds
Description | Unit | Part No.
Base O-ring for 96-well plate manifold |  | 5185-5779
Collection plate spacer for Agilent 1 mL deep-well, 12 mm |  | 5185-5775
Collection plate spacer for microtiter plate and Agilent 0.5 mL shallow well plate, 29 mm |  | 5185-5781
Collection plate spacer for most industry-standard deep-well plates, 2 mm |  | 5185-5780
Disposable reservoir tray for 96-well manifold | 25/pk | 5185-5782
96-well vacuum manifold, base assembly only |  | 5185-5797
Lid for 96-fixed well vacuum manifold |  | 5185-5798
Lid gasket for 96-well plate manifold |  | 5185-5778
Luer adapters for 96-well flexible cartridge | 25/pk | 5185-5789
Needle valve for 96-well manifold |  | 5185-5783
On/off valve for 96-well manifold |  | 5185-5785
Vacuum gauge for 96-well manifold |  | 5185-5786
Vacuum outlet (Ni plated) for 96-well manifold |  | 5185-5784

Sealing Mats

Sealing mats help prevent sample contamination or evaporation that can occur when plates are exposed to environmental conditions.

Collection plate, showing 96-position closing mat, 5042-1389

Sealing Mats
Description | Unit | Part No.
96-well plate sealing mats, round | 50/pk | 5042-1389

Product Index

Captiva Filtration

96-well Collection Plates and Cover

96-well Filter Kits

96-well Filter Plates

Captiva ND

96-well filter plates

Captiva NDLipids

96-well filter plates

Filter Cartridges

Sample Filtration

Disposable Syringes

Econofilters

Filtration Efficiency Study

Filtration Impact on LC Column Life

Layered Filters with Pre-Filter

Premium Syringe Filters

Selection Guide

Vacuum Collar

Chromatography Papers

Diatomaceous Earth Sorbents

Chem Elut

Hydromatrix

Plates

Tox Elut

Dried Matrix Spotting

Bond Elut Dried Matrix Spotting (DMS)

QuEChERS

Ceramic Homogenizers

Dispersive Kits

Extraction Kits

Standard Operating Procedure

Standards

Sample Filtration

Disposable Syringes

Econofilters

Filtration Efficiency Study

Filtration Impact on LC Column Life

Layered Filters with Pre-Filter

Premium Syringe Filters

Selection Guide

Solid Phase Extraction (SPE)

Bond Elut Accessories

20 μm Polyethylene Frits for SPE Cartridges

96 Well Plate Vacuum Manifold

96-well Accessories

Adapter Caps for Gilson

ASPEC SPE Systems

Adapters

Empty SPE Cartridges

Empty SPE Cartridges with Two Frits

Luer Stopcocks

Sealing Mats

Vac Elut 12 Manifold

Vac Elut 20 Manifold Tall Glass Basin

Vac Elut 20 Vacuum

Extraction Manifolds

Vac Elut SPS 24 Manifold

Bond Elut Plexa Polymeric SPE

Bond Elut Plexa

Bond Elut Plexa PAX

Bond Elut Plexa PCX

General Protocol

Bulk SPE

Bondesil

Cross Reference of Comparable Phases

by Manufacturer

Disk SPE

Bond Elut SPEC

96-well Plates

Cartridges

Empore Disk SPE

Inorganic SPE

Bond Elut Alumina

Bond Elut Florisil

Bond Elut Sodium Sulfate

Drying Cartridges

Mega Bond Elut Flash

Micro-volume SPE

OMIX Tips

OMIX Tips and Plates for Robotic

Automation

Microextraction

Accessories

Fibers

Kits

Polymeric SPE

Mixed Mode

Bond Elut NEXUS and NEXUS WCX

Reversed Phase

Bond Elut ENV

Bond Elut LMS

Bond Elut PPL

Silica-Based SPE

Ion Exchange

Bond Elut CBA

Bond Elut DEA

Bond Elut PRS

Bond Elut PSA

Bond Elut SAX

Bond Elut SCX

Mixed Mode

Bond Elut AccuCAT

Bond Elut Certify

Bond Elut Certify II

Normal Phase (Polar)

Bond Elut CN-E

Bond Elut Diol (2OH)

Bond Elut NH2

Bond Elut SI

Reversed Phase

Bond Elut C1

Bond Elut C18

Bond Elut C18 EWP

Bond Elut C18 OH

Bond Elut C2

Bond Elut C8

Bond Elut CH

Bond Elut PH

Sorbent Specifications

Specialty SPE

Bond Elut Carbon

Bond Elut Cellulose

Bond Elut Mycotoxin

Bond Elut PBA

Bond Elut PCB

EnvirElut

Part Number Index

102818C

12102001

12102002

12102003

12102004

12102005

12102006

12102007

12102009

12102010

12102011

12102012

12102013

12102014

12102015

12102016

12102017

12102017T

12102017TB

12102018

12102019

12102020

12102023

12102024

12102025

12102025T

12102026

12102027

12102028

12102029

12102030

12102031

12102032

12102033

12102034

12102036

12102037

12102038

12102039

12102040

12102041

12102042

12102042C250

12102042C500

12102043

12102044

12102044T

12102046

12102047

12102048

12102049

12102050

12102051

12102051T

12102052

12102052T

12102053

12102058

12102058B

12102059

12102060

12102062

12102063

12102064

12102067

12102068

12102069

12102070

12102071

12102073

12102074

12102075

12102076

12102077

12102078

12102079

12102080

12102081

12102081T

12102082

12102083

12102084

12102085

12102087

12102088

12102089

12102090

12102093

12102094

12102095

12102096

12102097

12102098

12102099

12102100

12102105

12102106

12102107

12102108

12102109

12102115

12102117

12102118

12102119

12102124

12102125

12102127

12102129

12102136

12102137

12102139

12102144

12102145

12102148

12102157

12102158

12102159

12102167

12102201

12103100

12103101

12103102

12105002

12105003

12105004

12105005

12105006

12105012

12105013

12105014

12105015

12105016

12105021

12105023

12105024

12105025

12105026

12105027

12105028

12105029

12105030

12105031

12105032

12107206

12107301

12107303

12107601

12107603

12108206

12108301

12108303

12108601

12108603

12108603T

12109206

12109301

12109301T

12109303

12109601

12109603

12109610

12109703

12113001

12113003

12113005

12113009

12113010

12113011

12113012

12113013

12113014

12113016

12113017

12113018

12113024

12113025

12113027

12113028

12113029

12113031

12113032

12113033

12113035

12113036

12113037

12113038

12113039

12113040

12113041

12113042

12113043

12113048

12113049

12113050

12113051

12113052

12113054

12113063

12113067

12113068

12113071

12113075

12113100

12113101

12131001

12131003

12131004

12131005

12131007

12131008

12131009

12131010

12131011

12131012

12131013

12131014

12131015

12131016

12131017

12131018

12131019

12131020

12131021

12131022

12131023

12131024

12131033

12131034

12131035

12132004

12143105

12145002

12145004

12145007

12145010

12145012

12145029

12162028B

12162029B

12162037B

12162040B

12162041B

12162042B

12162044B

12162048B

12162049B

12162050B

12162051B

12162052B

12162054B

12165001B

12166001B

12166002B

12166008B

12166011B

12166012B

12166013B

12166014B

12166043B

12166044B

12166045B

12166046B

12166050B

12169610B

12198001

12198002

12198003

12198004

12198005

12198006

12198007

12198008

12198009

12198010

12198011

12198012

12198014

12198015

12198017

12213001

12213006

12213009

12213011

12213012

12213013

12213015

12213020

12213021

12213023

12213024

12213025

12213027

12213033

12213037

12213039

12213040

12213041

12213042

12213044

12213047

12213049

12213061

12213073

12214013

12214015

12214016

12214019

12216061

12219001

12221305

12221306

12234003

12234004

12234005

12234022

12234025

12234025C

12234027

12234028

12234030

12234031

12234034

12234038

12234041

12234100

12234101

12234102

12234103

12234104

12234105

12234501

12234502

12234504

12234505

12234506

12234507

12234510

12234511

12234517

12236104

12236108

12252201

12252202

12253101

12253102

12253103

12255001

12255002

12255011

12255012

12255014

12255021

12255022

12256001

12256002

12256003

12256004

12256005

12256007

12256008

12256009

12256010

12256011

12256012

12256013

12256014

12256015

12256018

12256020

12256021

12256022

12256023

12256024

12256026

12256028

12256029

12256030

12256031

12256032

12256034

12256036

12256037

12256038

12256039

12256040

12256042

12256043

12256044

12256045

12256046

12256047

12256051

12256053

12256054

12256055

12256058

12256059

12256060

12256066

12256067

12256068

12256069

12256070

12256071

12256072

12256073

12256074

12256075

12256076

12256077

12256078

12256079

12256080

12256081

12256086

12256087

12256130

12256140

12256145

12256146

12256146TJ

12257506

12258506

12259506

12272001

12272002

12272004

12272005

12272007

12272020

12281001

12281002

12281024

12281101

12281102

12281305

12281306

12282001

12282002

12282003

12282004

12282005

12282006

126414

126418

14102001

14102005

14102010

14102013

14102016

14102017

14102025

14102028

14102032

14102037

14102040

14102041

14102043

14102044

14102051

14102052

14102058

14102062

14102068

14102075

14102076

14102078

14102079

14102080

14102081T

14102083

14102084

14102085

14102088

14102093

14113001

14113005

14113010

14113013

14113024

14113027

14113031

14113036

14113039

14113040

14113042

14113043

14113050

14113051

14113052

14113054

14113055

14213012

14213013

14213021

14213039

14256001

14256004

14256008

14256011

14256012

14256013

14256015

14256018

14256019

14256020

14256021

14256026

14256027

14256029

14256031

14256034

14256035

14256037

161618G

161622G

161632G

161818G

161822G

161832G

164632G

165022G

165032G

167022G

167232G

167816G

167822G

168016G

198003

198004

221032B

2264265032

327832

3664325032

391896301

391896302

391896303

391896304

391896306

391896307

391896308

391896309

391896313

391896314

391896315

391896316

391896401

392548402

392609902

446424

466430

5042-1389

5062-8534

5133000

5133005

5133007

5133008

5133009

5185-5775

5185-5778

5185-5779

5185-5780

5185-5781

5185-5782

5185-5783

5185-5784

5185-5785

5185-5786

5185-5789

5185-5797

5185-5798

5190-0499

5190-0500

5190-0501

5190-0502

5190-0503

5190-5082

5190-5083

5190-5084

5190-5085

5190-5086

5190-5087

5190-5088

5190-5091

5190-5092

5190-5093

5190-5094

5190-5095

5190-5096

5190-5097

5190-5098

5190-5099

5190-5106

5190-5107

5190-5108

5190-5109

5190-5110

5190-5111

5190-5116

5190-5117

5190-5120

5190-5122

5190-5126

5190-5127

5190-5128

5190-5129

5190-5132

5190-5133

5190-5134

5190-5135

5190-5261

5190-5262

5190-5263

5190-5264

5190-5265

5190-5266

5190-5267

5190-5268

5190-5269

5190-5270

5190-5271

5190-5272

5190-5273

5190-5274

5190-5275

5190-5276

5190-5277

5190-5278

5190-5279

5190-5280

5190-5281

5190-5282

5190-5283

5190-5284

52102002

52102017

52102026

52102051

52102081

52113050

52113051

52256014

5982-9100

5982-9101

5982-9102

5982-9103

5982-9104

5982-9105

5982-9106

5982-9107

5982-9108

5982-9109

5982-9110

5982-9111

5982-9112

5982-9113

5982-9114

5982-9115

5982-9116

5982-9117

5982-9311

5982-9312

5982-9313

65401507

7540101C

75401025

75401050

7540301C

75403025

7540302C

75403050

7540501C

75405050

7540701C

75408050

7540901C

75409050

75430260

7550101C

75501025

75501050

75502050

7550301C

75503050

7550701C

75507050

75508050

7550901C

75509025

75509050

7551701C

75530260

9301-6474

9301-6476

A120B12

A396011C

A3960125

A3960150

A396031C

A3960325

A3960350

A396051C

A3960525

A3960550

A396061C

A3960625

A3960650

A396071C

A3960725

A3960750

A396081C

A3960825

A3960850

A396091C

A3960925

A3960950

A3961010

A3961025

A396121C

A396151C

A3961525

A3961550

A3962060

A396291C

A3962925

A3962950

A3967010

A3967030

A3968010

A3968030

A3969010

A3969030

A400150

A400150K

A400150SK

A42001

A496011C

A4960125

A4960150

A496031C

A4960325

A4960350

A496041C

A4960425

A4960450

A496051C

A4960525

A4960550

A496061C

A4960625

A4960650

A496071C

A4960725

A4960750

A496091C

A4960925

A4960950

A4961010

A496111C

A4961150

A496121C

A496151C

A4962030

A496221C

A4962225

A4962250

A496291C

A496301C

A4963025

A4963050

A4964050

A4964150

A4967010

A4967030

A4968010

A4968030

A4969010

A4969030

A500401000

A5020535

A5020735

A5020770

A5021135

A5021170

A5021935

A5022570

A5060045

A5300002

A5300063

A5300635

A5307045

A5320130

A5320220

A5320230

A5320320

A5320330

A5320520

A5320530

A5320720

A5321020

A5321030

A5321120

A5321130

A5321920

A5321930

A5322020

A5322030

A532DAS

A532DAU

A5700310

A57003100

A57003100K

A5700310K

A57003MB

A57003MBK

A5700410

A57004100

A57004MB

A5700910

A57009100

A57009100K

A5700910K

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Ordering Information

Easy Ordering Terms and Conditions

Discounts and Delivery

Agilent Technologies specializes in fast delivery. In the US, if you call before 2 PM EST, we will ship your order that day. You may also request overnight express delivery before 6 PM EST and you will have your order the next day. Volume discounts on a variety of individual products are offered when the entire quantity is shipped to one address at one time.

A shipping and handling fee will be added to your order unless the purchase is over $2000 US for orders place online or over $4000 for orders place via phone. Special shipping (i.e., overnight in the US) is available in most regions at an additional cost.

Agilent is required to collect all state and local sales taxes unless the buyer's tax-exempt certificate is on file with Agilent Technologies. Please be prepared to provide a copy if it is not on file, when placing your order.

Please check with your Agilent Customer Service Representative, local Authorized Distributor, or the Agilent website for current prices, special offers, promotions and discounts when placing your order.

Satisfaction Guaranteed

If you are not satisfied with your Agilent product within the first 60 days, you may return your purchase in its original condition for a full refund or credit. A return policy statement is included in every Agilent shipment and posted under Product Information on the website. In the US and Canada, please call for a Return Authorization form and return instructions at 1-800-227-9770. If your Agilent product was purchased from a distributor, please contact the distributor.

Shipping Damages

If items are damages in transit, please follow the instructions below:

• If a shipment is visibly damaged upon arrival, do not accept it until the person making the delivery has endorsed the bill of lading with statement for the extent of the damage.

• If any damage is found after unpacking, retain all cartons and inner packaging and immediately request an inspection from the carrier.

• Notify the Agilent Customer Contact Center at 1-800-227-9770 about the damaged shipment so that we can make the appropriate sales adjustment and/or provide you with return instructions (Sales order number, product number and quantity damaged will be needed).

Easy Ways To Order

• Phone: 1-800-227-9770 (option 1, 1) in the US and Canada – Mon-Fri, 8AM to 8PM EST

• Fax: 1-302-633-8901 in the US

• Email: cag_sales-na@agilent.com in the US and Canada

• Online: www.agilent.com/chem in the US and Canada

Payment Options

• In the US, Visa, MasterCard, Discover and American Express are accepted with a minimum order of $20 (not applicable in all countries).

• Email ePay@agilent.com to make an electronic payment using the ACH/EFT (Automated Clearing House/Electronic Funds Transfer) method.

• Establish a charge account through your Agilent Customer Service Representative or Your Local Agilent sales office. An account number will be assigned to you for charging your purchases. Payment terms are net 30 days from the invoice date. All orders are subject to credit approval.

We will be happy to supply a price quote via, phone, email or fax if you need it in writing.

Warranties

All Agilent Technologies products in this catalog are designed and manufactured to stringent standards under the Agilent quality system registered to ISO 9001. At Agilent, we back every product with a 90-day warranty and a money-back guarantee. If Agilent receives notice of defects during the warranty period. Agilent shall, at its option, either repair or replace products which prove to be defective. If Agilent is unable, within a reasonable time, to repair or replace any product to a condition as warranted, the buyer shall be entitled to a refund of the purchase price upon return of the product to Agilent. The warranty period for each product begins on the day of shipment.

This warranty shall not apply to any defect, failure, or damage caused by improper use or improper or inadequate maintenance or care. This warranty is exclusive and no other warranty, whether written or oral, is expressed or implied. Agilent specifically disclaims the implied warranties of merchantability and fitness for particular purposes. The remedies provided herein are the buyer's sole and exclusive remedies. In no event shall Agilent be liable for direct, indirect, special, incidental, or consequential damages (including loss of profits) whether based on contract, tort, or any other legal theory.

NOTES