ABSTRACT									The nematode C. elegans has been used as a model for aging because of its limited lifespan and excellent genetic tools. Additional biochemical tools, however, are thinly spread, precluding full physiological work-up. Using diverse genetic models, including the caloric restriction mutant eat-2, the mitochondrial mutants mev-1 (succinate dehydrogenase) and cco-1 (cytochrome c oxidase), and pharmacological interventions, we established a variety of biochemical assays to complement lifespan analysis. These include the measurement of NAD+, mitochondrial respiration, oxidative stress, and mitochondrial morphology. In this webinar, we will discuss these different techniques, focusing in particular on the technical aspects of using Seahorse XF Extracellular Flux Analyzers for C. elegans respiration analysis.
IN THIS WEBINAR WE WILL DISCUSS									How metabolism connects to aging and lifespan How to analyze metabolic activity in the C. elegans How to use Seahorse XF Analyzers for the analysis of C. elegans respiration
RECORDED									November 30, 2011
TYPE									On Demand
LANGUAGE									English
									For Research Use Only. Not for use in diagnostic procedures.

	PRESENTED BY
		Laurent Mouchiroud, PhD École Polytechnique Fédérale de Lausanne
		Riekelt H. Houtkooper, PhD École Polytechnique Fédérale de Lausanne
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