Reactive oxygen and nitrogen species are part of normal metabolism and play prominent roles in cardiovascular (patho) physiology. Interestingly, these reactive species or their secondary products covalently modify proteins, thereby modulating enzyme function or eliciting redox signaling. Mitochondria, in particular, are prominent sources of such reactive species and are hotspots for oxidative protein modifications. Nevertheless, how reactive species or the protein modifications they induce affect cellular bioenergetics remains unclear. Accordingly, we have used extracellular flux (XF) analysis to understand how reactive species affect cellular bioenergetics.
IN THIS WEBINAR WE WILL DISCUSS
The different oxidative modifications occurring in the cell, including those that could impact cellular bioenergetics.
How the responses of cardiovascular cells to reactive species can differ based on cell type.
How oxidative modifications derived from reactive lipids and ROS/RNS affect bioenergetic function, reserve capacity, and the apparent respiratory state.
ASSAY(S)
Mitochondrial Function
RECORDED
January 20, 2010
TYPE
On Demand
LANGUAGE
English
For Research Use Only. Not for use in diagnostic procedures.
PRESENTED BY
Brad Hill, PhD
Center for Diabetes and Obesity Research, University of Louisville
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