Agilent Technologies introduces Nanoflow Proteomics Solution for protein identification and characterization

PALO ALTO, Calif., June 3, 2002

Agilent Technologies Inc. (NYSE: A) today introduced the Agilent Nanoflow Proteomics Solution, a highly integrated liquid chromatograph/ion trap mass spectrometer system optimized for proteomics applications such as the identification and characterization of proteolytically digested proteins. The Nanoflow Proteomics Solution is optimized to operate effectively and reliably at the nanoliter-per-minute flow rates that yield better LC/MS/MS sensitivity.

Automated Sample Cleanup and Enrichment

One of the many challenges of protein identification and characterization is the dilute nature of samples derived from in-gel digests. The Nanoflow Proteomics Solution provides fully automated desalting and enrichment thanks to integrated column switching valves and enrichment and analytical columns specially designed for nanoliter flow rates.

Superior Flow Control Enhances Performance

The Nanoflow Proteomics Solution features a new nanoflow LC pump optimized for nanoliter-per-minute flows. The nanoflow pump features active-feedback flow control that, unlike passive splitting, provides extremely stable and accurate flow control. Stable LC flow results in more stable ion generation for higher MS sensitivity. Active feedback automatically compensates for partial blockages and the real-time display of flow rate and pressure greatly simplifies troubleshooting.

Extremely Sensitive Detection

Fed by ultrastable nanoliter LC flow, the Agilent 1100 Series LC/MSD Trap SL ion trap mass spectrometer provides low-femtomole-level or better sensitivity. An excellent combination of scan speed and resolution means the LC/MSD Trap SL can often acquire multiple scans over even narrow chromatographic peaks, allowing spectrum averaging that increases data quality. SmartFrag collision-energy ramping ensures every peptide receives the correct collision energy for dissociation during MS/MS data acquisition.

Data-Dependent Acquisition and Analysis Yields Better Database Matches

Advanced second-generation, data-dependent software increases both the quality and quantity of MS/MS data acquired. High-quality data enhance the likelihood of achieving good, high-coverage protein database matches. Data-dependent acquisition and analysis functions of particular interest to proteomics researchers include the following:

  • active exclusion;
  • isotopic exclusion;
  • N most abundant precursors;
  • preferred charge-state selection; and
  • automatic spectral identification and averaging.

The Nanoflow Proteomics Solution software is fully compatible with the popular Mascot protein database search program from Matrix Science, Ltd. The entire analysis, from sample injection to database searching and report generation, can be automated.

U.S. Pricing and Availability

The price of the Nanoflow Proteomics Solution will start at approximately $260,000. Shipments are expected to begin in late summer, 2002.

Information about Agilent's life science products and services is available at

About Agilent Technologies

Agilent Technologies Inc. (NYSE: A) is a global technology leader in communications, electronics and life sciences. The company's 37,000 employees serve customers in more than 120 countries. Agilent had net revenue of $8.4 billion in fiscal year 2001. Information about Agilent is available on the Web at

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David Fisher
+1 650 485 4688

Doug Forsyth
+1 650 485 5603