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Update recommendations sequencing coverage

Multiplicom provides your laboratory with a broad range of molecular diagnostics solutions, which guide your laboratory towards the most reliable results. To make sure our solutions help you analyze the genes that matter in a simple, efficient and affordable manner, we continuously improve our products. As a result, we were able to reduce the recommended sequencing coverage for our germline and somatic MASTR RUO assays for Illumina NGS systems, such as MiSeq®, NextSeq® and MiniSeq®.

In order to make your MiSeq® sequencing runs more efficiently, we added Illumina version 3 flow cells (up to 2 times 300 cycles and generating more reads per run) for our somatic assays (tumor). Furthermore, based on experimental evidence we have lowered the recommended minimal coverage for Illumina sequencing for both germline and somatic assays (for SNV: from 50 to 20 reads per variant allele; for CNV: from 300 to 200 reads per amplicon).

Guidelines on minimal coverage per variant allele are pivotal to allow uniform calculations of the advised coverage per sample over all our MASTR products. Reducing the minimal required coverage depth for detection of SNV and CNV variants increases the capacity for including more samples on Illumina NGS platforms. These improved recommendations for our RUO assays make your run more affordable.

Multiplicom recommends the following updated minimal coverage per allele:

Minimal coverageGermline MASTRs 
SNV (per allele)
CNV
(per amplicon)
Somatic MASTRs
SNV (per allele)
CNA
(per amplicon)
Illumina NGS Systems2020020NA
Ion PGM System5030050NA

SNV = single nucleotide variant
CNV = copy number variation
CNA = copy number aberration
SRA = Short Read Amplification kit
NA = not applicable

Experts at your service

Our experts are always there to answer your questions by phone or by email. Our Field Application Specialists help you solve problems at your site, train your staff and advise you on how to improve your performance. They do not only know our products inside out, they also have an in-depth knowledge of next-generation sequencing and they understand how your business works. On top, they have a direct link with our R&D department, so that your questions and needs are continuously fed into our product innovations.

Don’t hesitate to contact our Expert support team if you have any further questions regarding our online web tools.