These 2nd generation visualization kits are for use with both rabbit and mouse primary antibodies. The biotinylated link antibody in the kits is produced in goat. No blocking step for reducing background staining caused by protein-protein interaction is required, the enzyme-conjugated streptavidin is provided in prediluted form.
It is a prerequisite for omission of the blocking step that the primary antibody is diluted in a buffer containing 1% bovine serum albumin. In K0609, the biotinylated link antibody shows no cross-reaction with rat immunoglobulins. This kit is therefore well-suited for use on rat tissue.
Note: The kits, K0609 and K675, do not contain chromogenic substrate.