GFAP isolated from bovine spinal cord has been used for immunization. Z0334 reacts strongly with human GFAP and with GFAP in many animal species tested: cat, cow, dog, mouse, rat and sheep.
In the central nervous system Z0334 labels astrocytes and some groups of ependymal cells. In the peripheral nervous system Schwann's cells, satellite cells and enteric glial cells are labeled. Negative labeling is found in skin, connective tissue, lymphatic tissue, muscle, gastrointestinal tract, including liver and pancreas, kidney, ureter and bladder.
Z0334 is useful particularly for distinguishing cells of astrocytic origin in the central nervous system. The antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections.