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Traditional Medicines

Huang Qin Extract, (burdock root) is used in Traditional Chinese Medicine (TCM) and can be analyzed by HPLC. In both method development and sample analysis, HPLC for TCM can be time consuming. UHPLC with sub-2-um technology is the most popular liquid chromatography technology, with distinct superiority in separation and speed over traditional HPLC. In this note, a UHPLC method was optimized to analyze a huang qin extract injection using the Agilent 1290 Infinity LC system with Agilent ZORBAX RRHD 1.8 um column. The resulting cycle time was 15% less than the original method. The method was then transferred to an Agilent 1200 Series LC system. Both the UHPLC and the transferred HPLC method were more effective than the original HPLC method. The figure shows the optimized separation conditions on the Agilent 1290 Infinity LC system (UHPLC).