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Peptide Mapping

Accurate Solutions For Protein Characterization

Comprehensive protein characterization is crucial for maintaining the quality control of biotherapeutics. Peptide mapping is used to detect and monitor single amino acid changes, modifications, and degradation products.

Peptide mapping is an essential technique for studying the primary structure of proteins. For recombinant protein pharmaceuticals, peptide mapping is used for the initial proof of structure characterization. It is also employed for lot-to-lot identity testing in support of bioprocess development and clinical trials. Peptide mapping is also used for monitoring the genetic stability of recombinant cell lines. Recently peptide mapping has become a rapid and convenient analysis method, expanding its utility in process monitoring and other manufacturing applications.

Agilent Technologies offers a comprehensive portfolio of solutions for the highly accurate confirmation of protein sequence, identification of modifications, and routine protein fingerprint monitoring for QA/QC, with the use of liquid chromatography systems and columns, capillary electrophoresis, and LC/MS systems.