Reliable Protein Separation Based On Charge And Glycosylation States
Electrophoresis of proteins and peptides provides separation based on isoelectric point or charge states, size or a combination of these attributes. Capillary Electrophoresis (CE) is commonly used for intact protein separation based on charge states and for glycan analysis. CE can also be combined with mass spectrometry, and is often used as an orthogonal technique to LC/MS, including to perform peptide mapping. Simple electrophoresis techniques like SDS-PAGE are commonly used for protein stability testing and QC, at-line monitoring of protein expression, and ini protein purification optimization and monitoring.
Agilent offers several options for capillary electrophoresis and micro-fluidic electrophoresis of proteins, including the Agilent 7100 Capillary Electrophoresis system for protein characterization and for protein modification analysis when combined with time-of-flight mass spectrometry (TOF MS). The 2200 TapeStation and 2100 Bioanalyzer are proposed as replacement platforms for SDS-PAGE gels for monitoring protein fate throughout process development.