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Peptide Mapping

Accurate Solutions For Protein Characterization


Comprehensive protein characterization is crucial for the quality control of biotherapeutics. It includes powerful methods that detect and monitor single amino acid changes, modifications, and degradation products.

Peptide mapping is an essential technique for studying the primary structure of proteins. For recombinant protein pharmaceuticals, peptide mapping is used for the initial proof of structure characterization. It is also employed for lot-to-lot identity testing in support of bioprocess development and clinical trials. Peptide mapping is used as the current method of choice for monitoring the genetic stability of recombinant cell lines. Peptide mapping recently became much more rapid and convenient, expanding its utility for process monitoring and other applications.

Agilent Technologies offers a comprehensive portfolio of solutions for the highly accurate confirmation of protein sequence, identification of modifications, and routine protein fingerprint monitoring for QA/QC, using liquid chromatography systems and columns, capillary electrophoresis, and LC/MS systems.