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Eliminate data uncertainty

Focused data

The end of trial-and-error electrophoresis

Agilent 2100 Bioanalyzer System
Eliminate Data Uncertainty

With traditional gel electrophoresis, you have to tediously review molecular fragments lane by lane—a subjective process that is vulnerable to variability between analysts.

The Agilent 2100 Bioanalyzer system provides sizing, quantitation, and purity assessments for DNA, RNA, and protein samples. This proven system delivers:

  • Greater analytical accuracy and precision. High resolution separation of bands gives you ultimate confidence in your results.
  • More time for research. Automated electrophoresis, with standardized kits and assays, requires little hands-on work. It produces digital data resulting in quick and easy sample analysis with more time to focus on next steps.
  • Extreme versatility. With its numerous available assays, the 2100 Bioanalyzer system is appropriate for multiple synthetic biology steps and downstream experiments.
  • An established track record. In 15+ years, the Agilent 2100 Bioanalyzer system has been cited over 33,000 times in peer-reviewed articles—proving itself in multiple applications.

Want to learn more about the 2100 Bioanalyzer System and automated electrophoresis?

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On-Demand eSeminar

Eliminating Data Uncertainty: Automated Electrophoresis for Genome Editing Analysis

Learn how an automated electrophoresis platform can increase your productivity and accuracy for nucleic acid and protein analysis.

Register now

Want to learn more about the 2100 Bioanalyzer System and automated electrophoresis? Contact us

NEW! Agilent application note:
Agilent Integrated Solutions for Design, Synthesis and Quality Control of Guide RNA for CRISPR-Cas9 Genome Editing Workflows. Download now

Genome editing

CRISPR/Cas has rapidly evolved to become the most popular approach for genome editing. Guide RNA (gRNA) is a structure that binds and directs a Cas nuclease to a specific cleavage target allowing for efficient gene modification, a faster process than traditional cloning approaches. Automated electrophoresis is used to monitor transcription and function of guide RNAs.

Guide RNA analysis

gRNAs are commonly created by in vitro transcription and are verified for correct sizing and quantitation on the 2100 Bioanalyzer system.

Cleavage/mutation detection

It is advised to test gRNAs for function with targeted cleavage or mutation detection assays.

References:

Vouillot L et al. Comparison of T7E1 and Surveyor mismatch cleavage assays… G3 (2015)

Ranganathan V et al. Expansion of the CRISPR-Cas9 genome targeting space… Nature Communications (2014)

Vector assembly

Vector assembly is an essential step in many genome editing workflows. Modified genes or DNA fragments are inserted into vectors with desired features. Automated electrophoresis assists in the analysis of assembled vectors which are verified by restriction digestion analysis and/or sequencing.

Fragment analysis

Whether using traditional cloning methods or new technologies, such as CRISPR/Cas, DNA fragments (from PCR amplicons to restriction digests) require objective analysis for size and concentration determination.

Next-generation sequencing

Next-generation sequencing confirms the sequences of vectors created by gene editing methods. The 2100 Bioanalyzer system ensures that NGS libraries are optimized prior to sequencing runs.

Functional genomics—RNA analysis

After genome editing events, effects of modifications on gene and system function must be evaluated. The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Using intact RNA is a key element for successful microarray, qRT-PCR, and transcriptome sequencing analyses.

RNA quality control

The 2100 Bioanalyzer system objectively measures RIN (RNA Integrity Number)—a well established quality standard for RNA samples.

Functional genomics—protein analysis

After genome editing events, effects of modifications on gene and system function must be evaluated. The 2100 Bioanalyzer system lets you perform functional characterization of proteins and complexes on the very same instrument.

Protein expression/
purification

Automated electrophoresis of protein samples allows for evaluation of expression patterns and monitoring of protein isolation and purification, including impurity detection and optimization of purification protocols.

Protein characterization

Protein kits for the 2100 Bioanalyzer system enable characterization of protein samples from genome-modified organisms, including the analysis of reducing and non-reducing conditions and the detection of low-level contaminants/
proteins/peptides.

For Research Use Only. Not for use in diagnostic procedures.