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Seahorse Analyzers

Live-cell Metabolic Assay Platform for Life Science Research

Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. The instruments perform compound addition and mixing, label-free detection, and automatic calculation of OCR and ECAR in real time.

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For Research Use Only. Not for use in diagnostic procedures.

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Beyond Lactate: Measuring Glycolytic Rates in Live Cells with the Agilent Seahorse XF Glycolytic Rate Assay

Glycolysis is a critical process in both normal cellular function and diseases such as cancer. Cells readily switch between this energy producing pathway and mitochondrial respiration, depending on energy demand and nutrient availability. As such, knowing how much glycolysis is being used by cells at any moment in time is important for understanding the interaction between metabolic activity and cell function. When glycolysis proceeds to completion, lactate and protons are extruded from the cell. The Seahorse XF Glycolytic Rate Assay Kit measures the resulting acidification rates and accounts for mitochondrial acidification to deliver the most accurate acidification-based measurement available.

XF Assay Tutorial: A rapid, real-time assay to monitor T cell activation using the Agilent Seahorse XFp Analyzer

This tutorial describes a novel assay that measures immune cell activation from within minutes of activation throughout the fully developed activated phenotype in both a qualitative and quantitative manner. This provides a sensitive window of opportunity to identify and characterize attributes that can drive immune responses to desired outcomes.

Agilent Seahorse XF Technology Webinar Series

Find upcoming Agilent Seahorse XF Technology webinars and view recordings of past webinars on key topics in cell metabolism research and bioenergetics.

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Featured Literature

Cell Metabolism Assays for Cancer Research

Discover how to measure the key parameters of cancer metabolism including metabolic phenotypes and tumor microenvironments.

Cell Metabolism Assays for Omics Research

Discover how to implement cell metabolism assay solutions in genomics, proteomics and metabolomics research.