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A New Approach to Metabolomics

Using Seahorse XF Analyzers and Agilent Mass Spectrometers

Seahorse XF Analyzers and Agilent Mass Spectrometers present a new approach to cell metabolism research. This powerful combination is enabling life science researchers to examine cellular metabolic pathways, and obtain unprecedented insight into cell function; as well as the impact of genes, proteins, and compounds on metabolism.

What kinds of information can I get?

XF Technology Untargeted Mass Spectrometry Targeted Mass Spectrometry
  • Simultaneously measure both energy production pathways - mitochondrial respiration and glycolysis - in real time.
  • Discover differences across a broad range of metabolites in cell extracts.
  • Map metabolic fluxes through different pathways of interest.
  • Measure metabolic function in live cells.
  • Screen hundreds of metabolites within a focused set of conditions.
  • Quantify dozens of metabolites across multiple conditions.
  • Identify phenotypic changes and metabolic reprogramming in dozens of conditions at once.
  • Get a global view of metabolic reprogramming in cells at any given time.
  • Confirm identity of unknown metabolites.

Why use a combined approach?

For life science researchers needing to assess metabolic changes in their cell models, Seahorse XF technology provides a metabolic phenotype, in live cells, that helps to determine whether functional changes have taken place, and which pathway(s) may be affected. These results then inform and guide the design of the MS analysis to maximize the effectiveness of metabolomics efforts. The combination of Seahorse XF/Agilent mass spec-based metabolomics facilitates a more integrated view of metabolism in biological models.

Use a Seahorse XF Analyzer to:

  • Determine the relevant cellular metabolic pathway for your flux analysis.
  • Measure the functional impact on cell metabolism of genes, proteins, metabolites,
    and drug-induced changes.
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For Research Use Only. Not for use in diagnostic procedures.